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Flow cytometry may allow microscope-independent detection of holocentric chromosomes in plants

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F16%3A00087971" target="_blank" >RIV/00216224:14310/16:00087971 - isvavai.cz</a>

  • Result on the web

    <a href="http://dx.doi.org/10.1038/srep27161" target="_blank" >http://dx.doi.org/10.1038/srep27161</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1038/srep27161" target="_blank" >10.1038/srep27161</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Flow cytometry may allow microscope-independent detection of holocentric chromosomes in plants

  • Original language description

    Two chromosomal structures, known as monocentric and holocentric chromosomes, have evolved in eukaryotes. Acentric fragments of monocentric chromosomes are unequally distributed to daughter cells and/or lost, while holocentric fragments are inherited normally. In monocentric species, unequal distribution should generate chimeras of cells with different nuclear DNA content. We investigated whether such differences in monocentric species are detectable by flow cytometry (FCM) as (i) a decreased nuclear DNA content and (ii) an increased coefficient of variance (CV) of the G1 peak after gamma radiation-induced fragmentation. We compared 13 monocentric and 9 holocentric plant species. Unexpectedly, monocentrics and holocentrics did not differ with respect to parameters (i) and (ii) in their response to gamma irradiation. However, we found that the proportion of G2 nuclei was highly elevated in monocentrics after irradiation, while holocentrics were negligibly affected.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    EF - Botany

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/GA13-29362S" target="_blank" >GA13-29362S: Evolution of holocentric chromosomes</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2016

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Scientific Reports

  • ISSN

    2045-2322

  • e-ISSN

  • Volume of the periodical

    6

  • Issue of the periodical within the volume

    June

  • Country of publishing house

    GB - UNITED KINGDOM

  • Number of pages

    7

  • Pages from-to

    "nestrankovano"

  • UT code for WoS article

    000377001500001

  • EID of the result in the Scopus database