Flow cytometry may allow microscope-independent detection of holocentric chromosomes in plants
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F16%3A00087971" target="_blank" >RIV/00216224:14310/16:00087971 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1038/srep27161" target="_blank" >http://dx.doi.org/10.1038/srep27161</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/srep27161" target="_blank" >10.1038/srep27161</a>
Alternative languages
Result language
angličtina
Original language name
Flow cytometry may allow microscope-independent detection of holocentric chromosomes in plants
Original language description
Two chromosomal structures, known as monocentric and holocentric chromosomes, have evolved in eukaryotes. Acentric fragments of monocentric chromosomes are unequally distributed to daughter cells and/or lost, while holocentric fragments are inherited normally. In monocentric species, unequal distribution should generate chimeras of cells with different nuclear DNA content. We investigated whether such differences in monocentric species are detectable by flow cytometry (FCM) as (i) a decreased nuclear DNA content and (ii) an increased coefficient of variance (CV) of the G1 peak after gamma radiation-induced fragmentation. We compared 13 monocentric and 9 holocentric plant species. Unexpectedly, monocentrics and holocentrics did not differ with respect to parameters (i) and (ii) in their response to gamma irradiation. However, we found that the proportion of G2 nuclei was highly elevated in monocentrics after irradiation, while holocentrics were negligibly affected.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EF - Botany
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GA13-29362S" target="_blank" >GA13-29362S: Evolution of holocentric chromosomes</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Scientific Reports
ISSN
2045-2322
e-ISSN
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Volume of the periodical
6
Issue of the periodical within the volume
June
Country of publishing house
GB - UNITED KINGDOM
Number of pages
7
Pages from-to
"nestrankovano"
UT code for WoS article
000377001500001
EID of the result in the Scopus database
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