Comparison of DNA-based techniques for differentiation of production strains of ale and lager brewing yeast
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F16%3A00089649" target="_blank" >RIV/00216224:14310/16:00089649 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1111/jam.13116" target="_blank" >http://dx.doi.org/10.1111/jam.13116</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/jam.13116" target="_blank" >10.1111/jam.13116</a>
Alternative languages
Result language
angličtina
Original language name
Comparison of DNA-based techniques for differentiation of production strains of ale and lager brewing yeast
Original language description
Brewing yeasts are classified into two species—Saccharomyces pastorianus and Saccharomyces cerevisiae. Most of the brewing yeast strains are natural interspecies hybrids typically polyploids and their identification is thus often difficult giving heterogenous results according to the method used. We performed genetic characterization of a set of the brewing yeast strains coming from several yeast culture collections by combination of various DNA-based techniques. The aim of this study was to select a method for species-specific identification of yeast and discrimination of yeast strains according to their technological classification. PCR-RFLP of HIS4 gene and RAPD-PCR of genomic DNA are reliable and suitable methods for fast identification of yeast strains. RAPD-PCR with primer 21 is a fast and reliable method applicable for differentiation of brewing yeasts with only 35% similarity of fingerprint profile between the two main technological groups (ale and lager) of brewing strains.
Czech name
—
Czech description
—
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
—
Result continuities
Project
—
Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Applied Microbiology
ISSN
1364-5072
e-ISSN
—
Volume of the periodical
120
Issue of the periodical within the volume
6
Country of publishing house
US - UNITED STATES
Number of pages
13
Pages from-to
1561-1573
UT code for WoS article
000379534800011
EID of the result in the Scopus database
—