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Three-dimensional homology model of GlcNAc-TV glycosyltransferase

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F16%3A00089828" target="_blank" >RIV/00216224:14310/16:00089828 - isvavai.cz</a>

  • Result on the web

    <a href="http://dx.doi.org/10.1093/glycob/cww010" target="_blank" >http://dx.doi.org/10.1093/glycob/cww010</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1093/glycob/cww010" target="_blank" >10.1093/glycob/cww010</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Three-dimensional homology model of GlcNAc-TV glycosyltransferase

  • Original language description

    The enzyme UDP-N-acetylglucosamine: a-d-mannoside b-1-6 N-acetylglucosaminyltransferase V (GnT-V) catalyzes the transfer of GlcNAc from the UDP-GlcNAc donor to the a-1-6-linked mannose of the trimannosyl core structure of glycoproteins to produce the b-1-6-linked branching of N-linked oligosaccharides. b-1-6-GlcNAc-branched N-glycans are associated with cancer growth and metastasis. Therefore, the inhibition of GnT-V represents a key target for anti-cancer drug development. However, the development of potent and specific inhibitors of GnT-V is hampered by the lack of information on the three-dimensional structure of the enzyme and on the binding characteristics of its substrates. Here we present the first 3D structure of GnT-V as a result of homology modeling. Various alignment methods, docking the donor and acceptor substrates, and molecular dynamics simulation were used to construct seven homology models of GnT-V and characterize the binding of its substrates.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    CE - Biochemistry

  • OECD FORD branch

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2016

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Glycobiology

  • ISSN

    0959-6658

  • e-ISSN

  • Volume of the periodical

    26

  • Issue of the periodical within the volume

    7

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    15

  • Pages from-to

    757-771

  • UT code for WoS article

    000384766000009

  • EID of the result in the Scopus database