Real-time PCR versus digital PCR in diagnostics of food-borne parasites
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F16%3A00091069" target="_blank" >RIV/00216224:14310/16:00091069 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Real-time PCR versus digital PCR in diagnostics of food-borne parasites
Original language description
In recent years, an increased number of reported cases of human infections by parasitic agents present in foodstuffs is recorded in Europe. Among the dominant pathogens are documented protozoa - Giardia intestinalis and Toxoplasma gondii. Cysts of G. intestinalis can survive long term in a cool and moist environment and contaminate e.g. fruit and vegetables growing in soil. Whereas T. gondii is an obligate intracellular parasite of warm-blooded animals, which forms tissue cysts in nervous and muscle tissue of the intermediate host; during the meat processing might be contaminated also tissue of uninfected hosts. Moreover, meat inspection of T. gondii at the slaughterhouses is not legislatively established. The detection possibilities of G. intestinalis and T. gondii were tested using two molecular methods - quantitative real-time PCR (qPCR) and digital PCR (dPCR).
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů