Quantification of Toxoplasma gondii in tissue samples of experimentally infected goats by magnetic capture and real-time PCR

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F13%3A43871517" target="_blank" >RIV/62157124:16270/13:43871517 - isvavai.cz</a>
Alternative codes found
RIV/62157124:16170/13:43871517 RIV/62157124:16810/13:43871517 RIV/00027162:_____/13:#0000993
Result on the web
<a href="http://dx.doi.org/10.1016/j.vetpar.2012.11.016" target="_blank" >http://dx.doi.org/10.1016/j.vetpar.2012.11.016</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.vetpar.2012.11.016" target="_blank" >10.1016/j.vetpar.2012.11.016</a>

Result language
angličtina
Original language name
Quantification of Toxoplasma gondii in tissue samples of experimentally infected goats by magnetic capture and real-time PCR
Original language description
Undercooked meat containing tissue cysts is one of the most common sources of Toxoplasma gondii infection in humans. Goats are very susceptible to clinical toxoplasmosis, and especially kids are common food animals, thereby representing a risk for humaninfection. A sequence-specific magnetic capture method was used for isolation of T. gondii DNA from tissue samples from experimentally infected goat-kids and real-time PCR for the 529 bp repeat element allowed quantification of T. gondii DNA. The contamination level in different types of tissue and in two groups of goats euthanized 30 and 90 dpi was compared. The highest concentration of T. gondii DNA in both groups of goats was found in lung tissue, but only the higher parasite count in lung tissue compared to other organs in group A (euthanized 30 dpi) was statistically significant. T. gondii concentrations were higher in liver and dorsal muscle samples from goats euthanized 90 dpi than in goats euthanized at 30 dpi, while the T. gond
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
GJ - Diseases and animal vermin, veterinary medicine
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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