Classification of Aeromonas spp. isolated from water and clinical sources and distribution of virulence genes
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F16%3A00091233" target="_blank" >RIV/00216224:14310/16:00091233 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1007/s12223-016-0464-9" target="_blank" >http://dx.doi.org/10.1007/s12223-016-0464-9</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s12223-016-0464-9" target="_blank" >10.1007/s12223-016-0464-9</a>
Alternative languages
Result language
angličtina
Original language name
Classification of Aeromonas spp. isolated from water and clinical sources and distribution of virulence genes
Original language description
In this work, 84 isolates of aeromonads were isolated from water and clinical samples, identified, and characterized. Identification was based on routine phenotyping combined with multiplex PCR. In this study, multiplex PCR was retested and reevaluated and its identification key was enhanced by 17 newly described species and five subspecies. Identification score increased from 36 % (only phenotyping) to 90% when supported with multiplex PCR. Further description of isolates included detection of eight virulence genes. These genes were overall present in 46 % (act), 2.4 % (ast), 80%(alt), 40%(ahh1), 20%(asa1), 69%(pla/lip/lipH3/alp- 1), 69 % (ser), and 81 % (fla), and no significant differences between water and clinical isolates were found. Results of this work show that the proper combination of different approaches is necessary for final identification of Aeromonas spp. at the species level.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/EE2.3.20.0183" target="_blank" >EE2.3.20.0183: Center of Experimental Biomedicine</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Folia Microbiologica
ISSN
0015-5632
e-ISSN
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Volume of the periodical
61
Issue of the periodical within the volume
6
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
9
Pages from-to
513-521
UT code for WoS article
000386351700009
EID of the result in the Scopus database
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