Plasmid-mediated colistin resistance in human and animal isolates of Escherichia coli: genomic surveillance

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F21%3A43879731" target="_blank" >RIV/62157124:16270/21:43879731 - isvavai.cz</a>
Alternative codes found
RIV/62157124:16810/21:43879731
Result on the web
<a href="https://fvhe.vfu.cz/files/upload/KMVP%202021_Sborn%C3%ADk.pdf" target="_blank" >https://fvhe.vfu.cz/files/upload/KMVP%202021_Sborn%C3%ADk.pdf</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Plasmid-mediated colistin resistance in human and animal isolates of Escherichia coli: genomic surveillance
Original language description
Increasing occurrence of colistin-resistant bacteria represents a global concern since colistin is lastline antibiotic for the treatment of severe infections. Recent discovery of mobile colistin resistance mediated by mcr genes has raised extensive concerns worldwide. Within national surveillance of mcr genes in human clinical isolates in the Czech Republic a total of 1440 colistin-resistant isolates (MIC >2 mg/l) were examined. At the same time more than 400 samples from food and food animals at slaughter were collected and selected for resistant isolates on agar media with colistin. The presence of mcr-1 to mcr-9 was tested by multiplex polymerase chain reaction (PCR). Isolates carrying mcr genes were subjected to antibiotic susceptibility testing and whole-genome sequencing to determined their resistance phenotype and genomic profiles. Conjugation experiments were used to test the transferability of mcr-carrying plasmids. The results showed a low prevalence of mcr genes in clinical samples (69/1440; 4,7 %) compared to isolates from veterinary sector (52/400; 13 %). Most isolates showed multi-drug resistance phenotype, belonged to various sequence types and carried mcr genes on conjugative plasmids.
Czech name
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Czech description
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Project
<a href="/en/project/NV18-09-00605" target="_blank" >NV18-09-00605: Enterobacteriaceae resistant to carbapenems and colistin: One-Health approach in addressing human and non-human sources of emerging antimicrobial resistance mechanisms</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
Sborník příspěvků XXIII. konference mladých vědeckých pracovníků s mezinárodní účastí
ISBN
978-80-7305-851-7
ISSN
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e-ISSN
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Number of pages
3
Pages from-to
114-116
Publisher name
Veterinární univerzita Brno
Place of publication
Brno
Event location
Brno
Event date
Jun 14, 2021
Type of event by nationality
EUR - Evropská akce
UT code for WoS article
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