National screening of plasmid-determined colistin-resistance mediated by mcr genes in human isolates in the Czech Republic

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F19%3A43878223" target="_blank" >RIV/62157124:16270/19:43878223 - isvavai.cz</a>

Alternative codes found

RIV/62157124:16810/19:43878223

Result on the web

—

DOI - Digital Object Identifier

—

Result language

angličtina

Original language name

National screening of plasmid-determined colistin-resistance mediated by mcr genes in human isolates in the Czech Republic

Original language description

Background: Increasing occurrence of colistin-resistant bacteria represents a global concern since colistin is lastline antibiotic used for the treatment of severe infections. Recent discovery of mobile colistin resistance mediated by mcr genes has raised extensive concerns worldwide. We aim to determine the prevalence of mcr genes in strains obtained from human clinical samples in the Czech Republic. Materials/methods: During 2008-2018, a total of 610 colistin-resistant isolates (MIC&gt;2mg/l) from various human clinical material were examined for the presence of mcr-1 to mcr-5 genes by multiplex PCR. Isolates carrying mcr genes were tested for susceptibility to 18 antimicrobials substances by the broth microdilution method and subjected to whole genome sequencing (WGS) using Miseq Illumina platform. Reads were assembled by SPAdes v3.12.0 in order to generate contigs that were used to identify antibiotic resistance genes, bacterial sequence types, and plasmid replicons using publicly available databases (ResFinder, MLSTFinder and PlasmidFinder). Transferability of mcr-carrying plasmids was determined by conjugation experiment followed by S1 pulse-field electrophoresis and DNA-DNA hybridization. Results: In total, eight mcr-1 (1,3%) and two mcr-4-positive isolates (0,3%) were obtained. Of eight mcr-1 carrying isolates, six were identified as Escherichia coli representing different sequence types (ST448, ST453, ST156, ST8186, ST1196 and ST744) and two isolates were identified as Klebsiella pneumoniae ST290 and ST147. Of two mcr-4 positive isolates, one was identified as Enterobacter cloaceae ST54 and another one as Acinetobacter baumanii ST747. Most isolates carried resistance genes to beta-lactams (n=7) and quinolones (n=6). The mcr-1 gene was mainly located on conjugative IncX4 plasmids that did not contain any additional antibiotic resistance genes, followed by multidrug-resistant IncHI2 and ColE10. Conclusions: Although this study demonstrated a low prevalence of mcr-positive isolates in patients in the Czech Republic, the occurrence of colistin-resistant bacteria should be considered as a possible risk for public health. This study became a baseline for national screening of plasmid-mediated colistin resistance that was initiated in 2018.

Czech name

—

Czech description

—

Type

O - Miscellaneous

CEP classification

—

OECD FORD branch

10606 - Microbiology

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů