Study of red clover (Trifolium pratense L.) genes specific for phytoalexin synthesis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F17%3A00094415" target="_blank" >RIV/00216224:14310/17:00094415 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Study of red clover (Trifolium pratense L.) genes specific for phytoalexin synthesis
Original language description
Phytoalexins are chemically diverse group of plant antimicrobial compounds, which are synthesized de novo by plants in response to biotic stress. Trifolirhizin is an isoflavonoid phytoalexin specific for red clover (Trifolium pratense L.). The aim of the study was experimental assessment of correlation between the expression of candidate genes and the level of trifolirhizin synthesized in the samples affected by biotic stress in comparison with control samples. Plant material included 4 red clover varieties (Tatra, Fresko, Tempus, Hungarotetra) and newly bred genotypes ( PO..) with different level or resistance to fungus Erysiphe trifolii. Three different types of stresses were applied.Computational analysis comprised in silico identification of four candidate genes for enzymes of biosynthetic pathway: phenylalanine ammonia lyase (PAL; EC4.3.1.5), chalcone synthase (CHS, EC 2.3.1.74), cinnamate 4-monooxygenase (CYP; EC 1.14.13.11) and 2'-hydroxyisoflavone reductase (IFR; EC 1.3.1.45). The analysis of gene PAL revealed increased expression in three plants influenced by pathogen infection and in one plant influenced by cutting off the leaves.
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
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OECD FORD branch
10603 - Genetics and heredity (medical genetics to be 3)
Result continuities
Project
<a href="/en/project/TH02010351" target="_blank" >TH02010351: The effective biotechnology for population chip synthesis as well as its verification on the pilot plant material matrix</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů