Genes associated with biological nitrogen fixation efficiency identified using RNA sequencing in red clover (Trifolium pratense L.)
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F26296080%3A_____%2F22%3AN0000124" target="_blank" >RIV/26296080:_____/22:N0000124 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14310/22:00127900
Result on the web
<a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9785344/" target="_blank" >https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9785344/</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/life12121975" target="_blank" >10.3390/life12121975</a>
Alternative languages
Result language
angličtina
Original language name
Genes associated with biological nitrogen fixation efficiency identified using RNA sequencing in red clover (Trifolium pratense L.)
Original language description
Commonly studied in the context of legume–rhizobia symbiosis, biological nitrogen fixation (BNF) is a key component of the nitrogen cycle in nature. Despite its potential in plant breeding and many years of research, information is still lacking as to the regulation of hundreds of genes connected with plant–bacteria interaction, nodulation, and nitrogen fixation. Here, we compared root nodule transcriptomes of red clover (Trifolium pratense L.) genotypes with contrasting nitrogen fixation efficiency, and we found 491 differentially expressed genes (DEGs) between plants with high and low BNF efficiency. The annotation of genes expressed in nodules revealed more than 800 genes not yet experimentally confirmed. Among genes mediating nodule development, four nod-ule-specific cysteine-rich (NCR) peptides were confirmed in the nodule transcriptome. Gene duplication analyses revealed that genes originating from tandem and dispersed duplication are significantly over-represented among DEGs. Weighted correlation network analysis (WGCNA) organized expression profiles of the transcripts into 16 modules linked to the analyzed traits, such as nitrogen fixation efficiency or sample-specific modules. Overall, the results obtained broaden our knowledge about transcriptomic landscapes of red clover’s root nodules and shift the phenotypic description of BNF efficiency on the level of gene expression in situ.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40101 - Agriculture
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Life-Basel
ISSN
2075-1729
e-ISSN
2075-1729
Volume of the periodical
12
Issue of the periodical within the volume
12
Country of publishing house
CH - SWITZERLAND
Number of pages
26
Pages from-to
1975
UT code for WoS article
000902695600001
EID of the result in the Scopus database
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