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Hyaluronidase reaction kinetics evaluated by capillary electrophoresis with UV and high-resolution mass spectrometry (HRMS) detection

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F17%3A00094585" target="_blank" >RIV/00216224:14310/17:00094585 - isvavai.cz</a>

  • Result on the web

    <a href="http://dx.doi.org/10.1016/j.aca.2016.11.036" target="_blank" >http://dx.doi.org/10.1016/j.aca.2016.11.036</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1016/j.aca.2016.11.036" target="_blank" >10.1016/j.aca.2016.11.036</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Hyaluronidase reaction kinetics evaluated by capillary electrophoresis with UV and high-resolution mass spectrometry (HRMS) detection

  • Original language description

    A capillary electrophoresis (CE) method with enzymatic reaction inside the capillary was developed for the study of hyaluronidase activity. Capillary electrophoresis was used for the first time for the determination of the kinetic constants (Km, Vmax and IC50) of the enzymatic degradation of hyaluronic acid. Km and Vmax were determined using CE with UV and MS detection. Reaction products were identified using CE-MS. IC50 of a standard natural inhibitor, epigallocatechin gallate, was also determined by CE-UV and CE-MS. Kinetic constant values obtained by CE compared well with literature which validated the developed CE-based assay. Homemade oligosaccharides were tested to search for new hyaluronidase inhibitors (or activators). Sulfatation in 6- and 4- position on chondroitin tetrasacharides were found to inhibit hydrolysis of hyaluronic acid. Small modification of one hydroxyl group in a trisaccharide compound was shown to be enough for changing effect on hyaluronidase activity. CE-based assay can be used for the screening of natural and synthetic inhibitors of hyaluronidase activity for cosmetic and therapeutic applications.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10600 - Biological sciences

Result continuities

  • Project

    <a href="/en/project/GBP206%2F12%2FG014" target="_blank" >GBP206/12/G014: Center for advanced bioanalytical technologies</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Others

  • Publication year

    2017

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Analytica Chimica Acta

  • ISSN

    0003-2670

  • e-ISSN

  • Volume of the periodical

    951

  • Issue of the periodical within the volume

    January

  • Country of publishing house

    NL - THE KINGDOM OF THE NETHERLANDS

  • Number of pages

    11

  • Pages from-to

    140-150

  • UT code for WoS article

    000392773800011

  • EID of the result in the Scopus database