Hyaluronidase reaction kinetics evaluated by capillary electrophoresis with UV and high-resolution mass spectrometry (HRMS) detection
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F17%3A00094585" target="_blank" >RIV/00216224:14310/17:00094585 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.aca.2016.11.036" target="_blank" >http://dx.doi.org/10.1016/j.aca.2016.11.036</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.aca.2016.11.036" target="_blank" >10.1016/j.aca.2016.11.036</a>
Alternative languages
Result language
angličtina
Original language name
Hyaluronidase reaction kinetics evaluated by capillary electrophoresis with UV and high-resolution mass spectrometry (HRMS) detection
Original language description
A capillary electrophoresis (CE) method with enzymatic reaction inside the capillary was developed for the study of hyaluronidase activity. Capillary electrophoresis was used for the first time for the determination of the kinetic constants (Km, Vmax and IC50) of the enzymatic degradation of hyaluronic acid. Km and Vmax were determined using CE with UV and MS detection. Reaction products were identified using CE-MS. IC50 of a standard natural inhibitor, epigallocatechin gallate, was also determined by CE-UV and CE-MS. Kinetic constant values obtained by CE compared well with literature which validated the developed CE-based assay. Homemade oligosaccharides were tested to search for new hyaluronidase inhibitors (or activators). Sulfatation in 6- and 4- position on chondroitin tetrasacharides were found to inhibit hydrolysis of hyaluronic acid. Small modification of one hydroxyl group in a trisaccharide compound was shown to be enough for changing effect on hyaluronidase activity. CE-based assay can be used for the screening of natural and synthetic inhibitors of hyaluronidase activity for cosmetic and therapeutic applications.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10600 - Biological sciences
Result continuities
Project
<a href="/en/project/GBP206%2F12%2FG014" target="_blank" >GBP206/12/G014: Center for advanced bioanalytical technologies</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Analytica Chimica Acta
ISSN
0003-2670
e-ISSN
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Volume of the periodical
951
Issue of the periodical within the volume
January
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
11
Pages from-to
140-150
UT code for WoS article
000392773800011
EID of the result in the Scopus database
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