The Oxidation Processes of Methylguanines on a Pencil Graphite Electrode

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F17%3A00096907" target="_blank" >RIV/00216224:14310/17:00096907 - isvavai.cz</a>

Result on the web

<a href="https://www.bestservis.eu/upload/file/Sbornik_metody17.pdf" target="_blank" >https://www.bestservis.eu/upload/file/Sbornik_metody17.pdf</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

The Oxidation Processes of Methylguanines on a Pencil Graphite Electrode

Original language description

In the last time, epigenetics is among top disciplines in the field of medicine research because epigenetic modifications of nucleic acids are associated with certain diseases and dysfunctions (e.g. human carcinomas – lung, thyroid, prostate and pancreas tumors-, leukemia, etc.). One of several epigenetic mechanisms that cells use to control gene expression is DNA methylation and one of different methylated guanines, N7- methyl G is recognized as the biomarker of DNA methylation. In the present study, electrochemical oxidation of methylated guanines (1N-, 7N- and 9N- derivatives) is studied to ascertain the relationship between the position of methyl group and the corresponding oxidation behavior. For this reason, the oxidation processes of methylguanines (methylGs) in the dependence on pH and the position of methyl group in the guanine molecule (pyrimidine ring vs. imidazole ring) were investigated. It was found that methylation has significant influence on the oxidation processes of methylGs and their oxidation is easier in the case of CH3- substitution at the pyrimidine ring than at imidazole one. Pencil graphite electrode (PeGE) was used as a working electrode throughout the study, since voltammetric signals of methylGs were poorly defined at conventional carbon electrodes, such as glassy carbon or HOPG.

Czech name

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Czech description

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Type

D - Article in proceedings

CEP classification

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OECD FORD branch

10403 - Physical chemistry

Project

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Continuities

S - Specificky vyzkum na vysokych skolach

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

XXXVII. Moderní elektrochemické metody

ISBN

9788090522152

ISSN

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e-ISSN

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Number of pages

4

Pages from-to

19-22

Publisher name

Srsenová Lenka - BEST servis Ústí nad Labem

Place of publication

Ústí nad Labem

Event location

Jetřichovice

Event date

May 15, 2017

Type of event by nationality

EUR - Evropská akce

UT code for WoS article

000432424300003