Monolithic capillary column with an integrated electrochemical detector

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F17%3A00113892" target="_blank" >RIV/00216224:14310/17:00113892 - isvavai.cz</a>

Alternative codes found

RIV/00216275:25310/17:39911330

Result on the web

<a href="https://www.sciencedirect.com/science/article/pii/S0021967317309251?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0021967317309251?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.chroma.2017.06.057" target="_blank" >10.1016/j.chroma.2017.06.057</a>

Result language

angličtina

Original language name

Monolithic capillary column with an integrated electrochemical detector

Original language description

The carbon fiber and silver microwire were used as working and pseudoreference electrode, respectively, and inserted into the ending of capillary to prepare monolithic capillary column with an integrated electrochemical detector. Prepared capillary devices offered stable and robust results with relative standard deviations of retention, resolution, and detection signal lower than 1.5, 5.5, and 5.0%, respectively. To further increase sensitivity of developed electrochemical microdetector, multiple pulse amperometry detection mode has been used. Optimized integrated device provided reliable chromatographic separation of mixture of neurotransmitters with calibration curve for dopamine linear from 0.5 to 20.0 mg L-1 and an instrumental limit of detection as low as 24 pg of injected dopamine. Finally, developed capillary column was applied to successful determination of dopamine in a human urine. By using both calibration curve and standard addition method, the dopamine level was determined to be 0.74 +/- 0.03 mgL(-1) and 0.71 +/- 0.02 mgL(-1), respectively. Triplicates of dopamine analysis provided relative standard deviations lower than 2.7% for intraday analyses, while interday relative standard deviations were lower than 3.6% for five consecutive days. (C) 2017 Elsevier B.V. All rights reserved.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10406 - Analytical chemistry

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Chromatography A

ISSN

0021-9673

e-ISSN

1873-3778

Volume of the periodical

1509

Issue of the periodical within the volume

AUG 2017

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

5

Pages from-to

171-175

UT code for WoS article

000407868800020

EID of the result in the Scopus database

2-s2.0-85021149318