An improved design to capture magnetic microparticles for capillary electrophoresis based immobilized microenzyme reactors
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F18%3A00100767" target="_blank" >RIV/00216224:14310/18:00100767 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1002/elps.201700434" target="_blank" >http://dx.doi.org/10.1002/elps.201700434</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/elps.201700434" target="_blank" >10.1002/elps.201700434</a>
Alternative languages
Result language
angličtina
Original language name
An improved design to capture magnetic microparticles for capillary electrophoresis based immobilized microenzyme reactors
Original language description
In this paper, we demonstrate the effectiveness of a new 3D printed magnet holder that enables capture of magnetic microparticles in commercially available capillary electrophoresis equipment with a liquid or air based coolant system. The design as well as the method to capture magnetic microparticles inside the capillary are discussed. This setup was tested at temperature and pH values suitable for performing enzymatic reactions. To demonstrate its applicability in CE- immobilized microenzyme reactors (IMER) development, human flavin-containing monooxygenase 3 and bovine serum albumin were immobilized on amino functionalized magnetic microparticles using glutaraldehyde. These microparticles were subsequently used to perform in-line capillary electrophoresis with clozapine as a model substrate.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10600 - Biological sciences
Result continuities
Project
<a href="/en/project/GA16-06106S" target="_blank" >GA16-06106S: Capillary electrophoresis based high-throughput system for beta-secretase inhibitor screening as a therapeutic target of Alzheimer's disease</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Electrophoresis
ISSN
0173-0835
e-ISSN
1522-2683
Volume of the periodical
2018
Issue of the periodical within the volume
7
Country of publishing house
DE - GERMANY
Number of pages
8
Pages from-to
981-988
UT code for WoS article
000429417500008
EID of the result in the Scopus database
2-s2.0-85040727781