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Monodisperse Carboxyl-Functionalized Poly(Ethylene Glycol)-Coated Magnetic Poly(Glycidyl Methacrylate) Microspheres: Application to the Immunocapture of beta-Amyloid Peptides

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F14%3A10283560" target="_blank" >RIV/00216208:11110/14:10283560 - isvavai.cz</a>

  • Alternative codes found

    RIV/61389013:_____/14:00434370

  • Result on the web

    <a href="http://dx.doi.org/10.1002/mabi.201400249" target="_blank" >http://dx.doi.org/10.1002/mabi.201400249</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1002/mabi.201400249" target="_blank" >10.1002/mabi.201400249</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Monodisperse Carboxyl-Functionalized Poly(Ethylene Glycol)-Coated Magnetic Poly(Glycidyl Methacrylate) Microspheres: Application to the Immunocapture of beta-Amyloid Peptides

  • Original language description

    Identification and evaluation of small changes in beta-amyloid peptide (A beta) levels in cerebrospinal fluid is of crucial importance for early detection of Alzheimer's disease. Microfluidic detection methods enable effective preconcentration of Ab using magnetic microparticles coated with Ab antibodies. Poly-(glycidyl methacrylate) microspheres are coated with alpha-amino-omega-methoxy-PEG(5000)/alpha-amino-omega-Boc-NH-PEG(5000) Boc groups deprotected and NH2 succinylated to introduce carboxyl groups. Capillary electrophoresis with laser-induced fluorescence detection confirms the efficient capture of A beta 1-40 peptides on the microspheres with immobilized monoclonal anti-A beta 6E10. The capture specificity is confirmed by comparing A beta 1-40 levels on the anti-IgG-immobilized particles used as a control.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    CD - Macromolecular chemistry

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/7E12053" target="_blank" >7E12053: Nanosystems for early Diagnosis of Neurodegenerative Diseases</a><br>

  • Continuities

    R - Projekt Ramcoveho programu EK

Others

  • Publication year

    2014

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Macromolecular Bioscience

  • ISSN

    1616-5187

  • e-ISSN

  • Volume of the periodical

    14

  • Issue of the periodical within the volume

    11

  • Country of publishing house

    DE - GERMANY

  • Number of pages

    10

  • Pages from-to

    1590-1599

  • UT code for WoS article

    000344749200009

  • EID of the result in the Scopus database