PEGylation controls attachment and engulfment of monodisperse magnetic poly(2-hydroxyethyl methacrylate) microspheres by murine J774.2 macrophages
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F17%3A00476589" target="_blank" >RIV/61389013:_____/17:00476589 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.apsusc.2017.07.148" target="_blank" >http://dx.doi.org/10.1016/j.apsusc.2017.07.148</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.apsusc.2017.07.148" target="_blank" >10.1016/j.apsusc.2017.07.148</a>
Alternative languages
Result language
angličtina
Original language name
PEGylation controls attachment and engulfment of monodisperse magnetic poly(2-hydroxyethyl methacrylate) microspheres by murine J774.2 macrophages
Original language description
The first objective of this work was to prepare biocompatible magnetic polymer microspheres with reactive functional groups that could withstand nonspecific protein adsorption from biological media. Carboxyl group-containing magnetic poly(2-hydroxyethyl methacrylate) (mgt.PHEMA) microspheres ~4 micro m in size were prepared by multistage swelling polymerization, precipitation of iron oxide inside their pores, and coating with an .alpha.-methoxy-.omega.-amino poly(ethylene glycol) (CH3O-PEG750-NH2 or CH3O-PEG5,000-NH2)/.alpha.-amino-.omega.-t-Boc-amino poly(ethylene glycol) (H2N-PEG5,000-NH-t-Boc) mixture. The mgt.PHEMA@PEG microspheres contained ~10 micro mol COOH per g. Biocompatibility of the particles was evaluated by their treatment with human embryonic kidney cells of the HEK293 line. The microspheres did not interfere with the growth of these cells, suggesting that the particles can be considered non-toxic. A second goal of this study was to address on the interaction of the developed microspheres with macrophages that commonly eliminate foreign microbodies appearing in organisms. Murine J774.2 macrophages (J774.2) were cultured in the presence of the neat and PEGylated microspheres for 2 h. Mgt.PHEMA@PEG5,000 microspheres significantly adhered to the surface of J774.2 macrophages but were minimally engulfed. Due to these properties, the mgt.PHEMA@PEG microspheres might be useful for application in drug delivery systems and monitoring of the efficiency of phagocytosis.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10404 - Polymer science
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Applied Surface Science
ISSN
0169-4332
e-ISSN
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Volume of the periodical
426
Issue of the periodical within the volume
31 December
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
10
Pages from-to
315-324
UT code for WoS article
000413658200035
EID of the result in the Scopus database
2-s2.0-85026406051