HotSpot Wizard 3.0: web server for automated design of mutations and smart libraries based on sequence input information
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F18%3A00101754" target="_blank" >RIV/00216224:14310/18:00101754 - isvavai.cz</a>
Alternative codes found
RIV/00159816:_____/18:00069364 RIV/00216305:26230/18:PU130747
Result on the web
<a href="http://dx.doi.org/10.1093/nar/gky417" target="_blank" >http://dx.doi.org/10.1093/nar/gky417</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1093/nar/gky417" target="_blank" >10.1093/nar/gky417</a>
Alternative languages
Result language
angličtina
Original language name
HotSpot Wizard 3.0: web server for automated design of mutations and smart libraries based on sequence input information
Original language description
HotSpot Wizard is a web server used for the automated identification of hotspots in semi-rational protein design to give improved protein stability, catalytic activity, substrate specificity and enantioselectivity. Since there are three orders of magnitude fewer protein structures than sequences in bioinformatic databases, the major limitation to the usability of previous versions was the requirement for the protein structure to be a compulsory input for the calculation. HotSpot Wizard 3.0 now accepts the protein sequence as input data. The protein structure for the query sequence is obtained either from eight repositories of homology models or is modeled using Modeller and I-Tasser. The quality of the models is then evaluated using three quality assessment tools-WHAT_CHECK, PROCHECK and MolProbity. During follow-up analyses, the system automatically warns the users whenever they attempt to redesign poorly predicted parts of their homology models. The second main limitation of HotSpot Wizard's predictions is that it identifies suitable positions for mutagenesis, but does not provide any reliable advice on particular substitutions. A new module for the estimation of thermodynamic stabilities using the Rosetta and FoldX suites has been introduced which prevents destabilizing mutations among pre-selected variants entering experimental testing.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Nucleic Acids Research
ISSN
0305-1048
e-ISSN
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Volume of the periodical
46
Issue of the periodical within the volume
W1
Country of publishing house
US - UNITED STATES
Number of pages
7
Pages from-to
„W356“-„W362“
UT code for WoS article
000438374100057
EID of the result in the Scopus database
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