Study of lectins from Photorhabdus luminescens bacterium
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F18%3A00103557" target="_blank" >RIV/00216224:14310/18:00103557 - isvavai.cz</a>
Result on the web
<a href="https://2018.febscongress.org/abstract_preview.aspx?idAbstractEnc=4424170093099099099100424170" target="_blank" >https://2018.febscongress.org/abstract_preview.aspx?idAbstractEnc=4424170093099099099100424170</a>
DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Study of lectins from Photorhabdus luminescens bacterium
Original language description
We present novel lectins from Photorhabdus luminescens with an unusual heptabladed beta-propeller fold. These proteins show very high sequence identity with recently described lectins PLL and PHL from the Photorhabdus genus. Furthermore, all lectins are localized in a row in a bacterial genome. Lectins, a group of proteins capable of binding glycoconjugates specifically and reversibly, are showing their importance in both mutualistic and parasitic interactions between microorganism and hosts. Both of these stages are present in the P. luminescens life cycle. The bacterium is highly pathogenic towards the larval stadium of various insect species and mutualistic with infective juveniles of the nematode Heterorhabditis bacteriophora. For structural and functional characterization of Photorhabdus lectins, the wide range of methods was used, e.g. glycan array, surface plasmon resonance, isothermal titration calorimetry, analytical ultracentrifugation, X-ray crystallography and biological assays performed with human blood and hemolymph (reactive oxygen species production and phenoloxidase activity). Whereas heptabladed beta-propeller fold is common for all studied lectins, the oligomeric state differs from mono- to di-mer. All studied lectins recognize L-fucose and O-methylated disaccharide glucose(1-4)rhamnose, but the fine binding specificities vary. The biological assay shows inhibition of the immune system response in the presence of P. luminescens lectins. Our results indicate these lectins might be involved in P. luminescens pathogenicity towards insect larva.
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
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OECD FORD branch
10700 - Other natural sciences
Result continuities
Project
<a href="/en/project/LQ1601" target="_blank" >LQ1601: CEITEC 2020</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů