Post rapid freezing growth of Antarctic strain of Heterococcus sp. monitored by cell viability and chlorophyll fluorescence
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F18%3A00104762" target="_blank" >RIV/00216224:14310/18:00104762 - isvavai.cz</a>
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S0011224018302001" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0011224018302001</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.cryobiol.2018.10.004" target="_blank" >10.1016/j.cryobiol.2018.10.004</a>
Alternative languages
Result language
angličtina
Original language name
Post rapid freezing growth of Antarctic strain of Heterococcus sp. monitored by cell viability and chlorophyll fluorescence
Original language description
The soil microalgae of the genus Heterococcus are found in cold environments and have been reported for the terrestrial ecosystems of several Sub-Antarctic and Antarctic Islands. This study focused on resistance of Heterococcus sp. to sub-zero temperature. Heterococcus sp. was isolated from soil samples from James Ross Island, Antarctica. Culture of Heterococcus sp. grown in liquid medium were used to study ribitol effects at sub-zero temperatures on the species resistance to rapid freezing (RF, immersion of a sample into liquid nitrogen) and consequent cultivation on agar. Before the experiment, Heterococcus sp. was cultured in liquid medium for 11 months and then treated in ribitol concentrations of 32 or 50 mM for 2h. Then, 1ml samples were frozen to minus 196°C in liquid nitrogen (day 0) and inoculated on BBM agar after thawing. Number of living and dead cells was evaluated and the cell viability (PV was calculated repeatedly using the optical microscopy approach. The addition of ribitol caused a noticable increase in PV on days 9, 12, 14 (with a PV of 25–45% in ribitol-treated samples compared to 10% in the untreated control). In the following period (d 16–19), the positive effect of ribitol on Pv was less pronounced but still statistically significant. To evaluate the negative effects of RF on chlorophyll fluorescence parameters, the potential yield of photochemical reactions in PS II (FV/FM), and the effective quantum yield of photochemical reactions in PS II (PhiPSII) were measured immediately before and after RF. Consequently, FV/FM and PhiPSII of agar inoculates were measured repeatedly for 30 d cultivation in 3 d interval. Both..
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10600 - Biological sciences
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Cryobiology
ISSN
0011-2240
e-ISSN
1090-2392
Volume of the periodical
85
Issue of the periodical within the volume
6
Country of publishing house
US - UNITED STATES
Number of pages
8
Pages from-to
39-46
UT code for WoS article
000454851300007
EID of the result in the Scopus database
2-s2.0-85055909602