The effect of shock freezing on physiological properties and consequent growth of Antarctic filamentous (Stigeoclonium sp.) and coccal alga (Diplosphaera chodatii) on agar plates
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F19%3A00109872" target="_blank" >RIV/00216224:14310/19:00109872 - isvavai.cz</a>
Result on the web
<a href="http://www.sci.muni.cz/CPR/17cislo/Orekhova_web.pdf" target="_blank" >http://www.sci.muni.cz/CPR/17cislo/Orekhova_web.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.5817/CPR2019-1-4" target="_blank" >10.5817/CPR2019-1-4</a>
Alternative languages
Result language
angličtina
Original language name
The effect of shock freezing on physiological properties and consequent growth of Antarctic filamentous (Stigeoclonium sp.) and coccal alga (Diplosphaera chodatii) on agar plates
Original language description
In this study, we investigated the effects of shock freezing on physiological properties and consequent growth of in the Antarctic alga Stigeoclonium sp. and comparative coccal alga Diplosphaera chodatii on agar plates. Culture of algae grown in liquid medium were used to study subzero temperatures on the species resistance to shock freezing. Then, microalgae were frozen in liquid nitrogen and inoculated on BBM agar after thawing. Physiological status of algae was evaluated by chlorophyll fluorescence parameters during 28 days. The results showed that interspecific differences existed in their tolerance to shock freezing, as well as their consequent growth rate on agars. Direct effects of freezing in liquid nitrogen was demonstrated in chlorophyll fluorescence parameters recorded immediately after thawing the samples (in liquid medium). In spite of the fact that majority of cells was destroyed by shock freezing, the potential of photochemical processes in PS II (FV/FM) remained constant in D. chodatii. It may indicate high resistance of the species to freezing/thawing cycles and a capability of the surviving cells, core chlorophylls in PS II respectively, to perform photosynthetic processes related to PS II. Contrastingly, Stigeoclonium sp. showed a shock freezing-dependent decrease in FV/FM. When shock-frozen, thawed and inoculated on agar plates, the culture of D. chodatii, and Stigeoclonium sp. showed cultivation time-dependent increase in chlorophyll fluorescence parameters (FV/FM, FS).
Czech name
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Czech description
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Classification
Type
J<sub>SC</sub> - Article in a specialist periodical, which is included in the SCOPUS database
CEP classification
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OECD FORD branch
10611 - Plant sciences, botany
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Czech Polar Reports
ISSN
1805-0689
e-ISSN
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Volume of the periodical
9
Issue of the periodical within the volume
1
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
12
Pages from-to
37-48
UT code for WoS article
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EID of the result in the Scopus database
2-s2.0-85079839504