The oxidation double peak of reduced guanine residues in short oligodeoxynucleotides: A study of its origin

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F18%3A00106524" target="_blank" >RIV/00216224:14310/18:00106524 - isvavai.cz</a>

Result on the web

<a href="https://doi.org/10.1016/j.jelechem.2018.03.013" target="_blank" >https://doi.org/10.1016/j.jelechem.2018.03.013</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jelechem.2018.03.013" target="_blank" >10.1016/j.jelechem.2018.03.013</a>

Result language

angličtina

Original language name

The oxidation double peak of reduced guanine residues in short oligodeoxynucleotides: A study of its origin

Original language description

The aim of this study is to find a deeper understanding of the long neglected oxidation double peak of reduced guanines (G(red)ODP) produced at a mercury electrode (ME) by the reverse oxidation of the guanine reduction product (G(red)). Although the reduction processes of G moieties at an ME are hidden in the cathodic currents of the background solvent decompositions, and the G reduction process thus cannot be examined directly, the G reduction product can be oxidized back to G, yielding an oxidation signal at about 0.2V. depending on the measurement conditions, the oxidation signal of Gred may assume the form of a single peak or a double peak with GI and GII. The cause of the double peak generation remains unknown. However, the novel approach based on combining elements of chronoamperometry and voltammetry proposes that the G(red)ODP originates from the oxidation of DNA fragments in two different adsorption conformations. The results indicate thet the orientation of G residues is controlled by the repulsive interaction after thier reduction and from the difference of GI and GII potentials the repulsion energy was estimated. The new findings may find application as a tool to indicate the structural and surface properties of short oligonucleotides with G bases

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)

Project

<a href="/en/project/LD15058" target="_blank" >LD15058: Preparation of substrates for Surface Enhanced Raman Spectroscopy using electrochemical, electrophoretic and spark discharge utilizing methods.</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Electroanalytical Chemistry

ISSN

1572-6657

e-ISSN

1873-2569

Volume of the periodical

821

Issue of the periodical within the volume

July

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

7

Pages from-to

104-110

UT code for WoS article

000437818600017

EID of the result in the Scopus database

2-s2.0-85046100800