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Pellet patented technology for fast and distinct visual detection of cholinesterase inhibitors in liquids

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F18%3A00110215" target="_blank" >RIV/00216224:14310/18:00110215 - isvavai.cz</a>

  • Alternative codes found

    RIV/62157124:16370/18:43876631 RIV/00027162:_____/18:N0000157

  • Result on the web

    <a href="https://www.sciencedirect.com/science/article/pii/S073170851830880X?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S073170851830880X?via%3Dihub</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1016/j.jpba.2018.08.050" target="_blank" >10.1016/j.jpba.2018.08.050</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Pellet patented technology for fast and distinct visual detection of cholinesterase inhibitors in liquids

  • Original language description

    The main objective of the presented research was to prepare an innovative carrier as a filler for detection tubes in the form of double-coated pellets with a very significant color transition during the detection of cholinesterase inhibitors such as nerve agents, organophosphorus or carbamate insecticides in liquids that is observable visually and also spectrophotometrically at 412 nm. The pellet cores were prepared by the extrusion/spheronization method. Consecutively, two different coats were applied on the pellet cores in the coating device using the Wurster column method. To increase the color change intensity, the second semipermeable coat based on Eudragit (R) RL was applied on top of the first coat, which was formed by butyrylcholinesterase immobilized in hydroxypropyl methylcellulose. Prepared samples differing in thickness of the second coat were evaluated for their quality parameters, enzymatic activity and inhibition. The detection mechanism was based on the standard Ellman's colorimetric reaction. It was observed that the semipermeable coat prevented leaching of the enzyme into the solution and led to an increased intensity of color transition from white - yellow to white - deep yellow/orange, thus enabling a more accurate visual detection. This system allows easy, rapid and safe identification of cholinesterase inhibitors in liquids, especially chemical warfare agents.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    30104 - Pharmacology and pharmacy

Result continuities

  • Project

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2018

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Journal of Pharmaceutical and Biomedical Analysis

  • ISSN

    0731-7085

  • e-ISSN

    1873-264X

  • Volume of the periodical

    161

  • Issue of the periodical within the volume

    August

  • Country of publishing house

    NL - THE KINGDOM OF THE NETHERLANDS

  • Number of pages

    8

  • Pages from-to

    206-213

  • UT code for WoS article

    000447682300023

  • EID of the result in the Scopus database

    2-s2.0-85052438910