Osteogenic Potential of Caspases Related to Endochondral Ossification
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F18%3A00111965" target="_blank" >RIV/00216224:14310/18:00111965 - isvavai.cz</a>
Alternative codes found
RIV/67985904:_____/18:00489550
Result on the web
<a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5761947/pdf/10.1369_0022155417739283.pdf" target="_blank" >https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5761947/pdf/10.1369_0022155417739283.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1369/0022155417739283" target="_blank" >10.1369/0022155417739283</a>
Alternative languages
Result language
angličtina
Original language name
Osteogenic Potential of Caspases Related to Endochondral Ossification
Original language description
Caspases have functions particularly in apoptosis and inflammation. Increasing evidence indicates novel roles of these proteases in cell differentiation, including those involved in osteogenesis. This investigation provides a complex screening of osteogenic markers affected by pan caspase inhibition in micromass cultures derived from mouse forelimbs. PCR Array analysis showed significant alterations in expression of 49 osteogenic genes after 7 days of inhibition. The largest change was a decrease in CD36 expression, which was confirmed at organ level by caspase inhibition in cultured mouse ulnae followed by CD36 immunohistochemical analysis. So far, available data point to osteogenic potential of pro-apoptotic caspases. Therefore, the expression of pro-apoptotic caspases (-3, -6, -7, -8, -9) within the growth plate of mouse forelimbs at the stage where the individual zones are clearly apparent was studied. Caspase-9 was reported in the growth plate for the first time as well as caspase-6 and -7 in the resting zone, caspase-7 in the proliferation, and caspase-6 and -8 in the ossification zone. For all caspases, there was a gradient increase in activation toward the ossification zone. The distribution of staining varied significantly from that of apoptotic cells, and thus, the results further support non-apoptotic participation of caspases in osteogenesis.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10601 - Cell biology
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY
ISSN
0022-1554
e-ISSN
1551-5044
Volume of the periodical
66
Issue of the periodical within the volume
1
Country of publishing house
GB - UNITED KINGDOM
Number of pages
12
Pages from-to
47-58
UT code for WoS article
000418835500004
EID of the result in the Scopus database
2-s2.0-85039734166