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Osteogenic Potential of Caspases Related to Endochondral Ossification

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16170%2F18%3A43877235" target="_blank" >RIV/62157124:16170/18:43877235 - isvavai.cz</a>

  • Result on the web

    <a href="https://journals.sagepub.com/doi/pdf/10.1369/0022155417739283" target="_blank" >https://journals.sagepub.com/doi/pdf/10.1369/0022155417739283</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1369/0022155417739283" target="_blank" >10.1369/0022155417739283</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Osteogenic Potential of Caspases Related to Endochondral Ossification

  • Original language description

    Caspases have functions particularly in apoptosis and inflammation. Increasing evidence indicates novel roles of these proteases in cell differentiation, including those involved in osteogenesis. This investigation provides a complex screening of osteogenic markers affected by pan caspase inhibition in micromass cultures derived from mouse forelimbs. PCR Array analysis showed significant alterations in expression of 49 osteogenic genes after 7 days of inhibition. The largest change was a decrease in CD36 expression, which was confirmed at organ level by caspase inhibition in cultured mouse ulnae followed by CD36 immunohistochemical analysis. So far, available data point to osteogenic potential of pro-apoptotic caspases. Therefore, the expression of pro-apoptotic caspases (-3, -6, -7, -8, -9) within the growth plate of mouse forelimbs at the stage where the individual zones are clearly apparent was studied. Caspase-9 was reported in the growth plate for the first time as well as caspase-6 and -7 in the resting zone, caspase-7 in the proliferation, and caspase-6 and -8 in the ossification zone. For all caspases, there was a gradient increase in activation toward the ossification zone. The distribution of staining varied significantly from that of apoptotic cells, and thus, the results further support non-apoptotic participation of caspases in osteogenesis.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10608 - Biochemistry and molecular biology

Result continuities

  • Project

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2018

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Journal of histochemistry &amp; cytochemistry

  • ISSN

    1551-5044

  • e-ISSN

  • Volume of the periodical

    66

  • Issue of the periodical within the volume

    1

  • Country of publishing house

    GB - UNITED KINGDOM

  • Number of pages

    12

  • Pages from-to

    47-58

  • UT code for WoS article

    000418835500004

  • EID of the result in the Scopus database

    2-s2.0-85039734166