Size-dependent genotoxicity of silver, gold and platinum nanoparticles studied using the mini-gel comet assay and micronucleus scoring with flow cytometry

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F18%3A00112173" target="_blank" >RIV/00216224:14310/18:00112173 - isvavai.cz</a>

Result on the web

<a href="https://watermark.silverchair.com/gex027.pdf?token=AQECAHi208BE49Ooan9kkhW_Ercy7Dm3ZL_9Cf3qfKAc485ysgAAAnIwggJuBgkqhkiG9w0BBwagggJfMIICWwIBADCCAlQGCSqGSIb3DQEHATAeBglghkgBZQMEAS4wEQQMam06LyYovadqWl7oAgEQgIICJRPfOeuz30abpTUXjpGo7yvPbJdBw170OjYb8PLkr_Q4W9o" target="_blank" >https://watermark.silverchair.com/gex027.pdf?token=AQECAHi208BE49Ooan9kkhW_Ercy7Dm3ZL_9Cf3qfKAc485ysgAAAnIwggJuBgkqhkiG9w0BBwagggJfMIICWwIBADCCAlQGCSqGSIb3DQEHATAeBglghkgBZQMEAS4wEQQMam06LyYovadqWl7oAgEQgIICJRPfOeuz30abpTUXjpGo7yvPbJdBw170OjYb8PLkr_Q4W9o</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/mutage/gex027" target="_blank" >10.1093/mutage/gex027</a>

Result language

angličtina

Original language name

Size-dependent genotoxicity of silver, gold and platinum nanoparticles studied using the mini-gel comet assay and micronucleus scoring with flow cytometry

Original language description

Metallic nanoparticles (NPs) are promising nanomaterials used in different technological solutions as well as in consumer products. Silver (Ag), gold (Au) and platinum (Pt) represent three metallic NPs with current or suggested use in different applications. Pt is also used as vehicle exhaust catalyst leading to a possible exposure via inhalation. Despite their use, there is limited data on their genotoxic potential and possible size-dependent effects, particularly for Pt NPs. The aim of this study was to explore size-dependent genotoxicity of these NPs (5 and 50 nm) following exposure of human bronchial epithelial cells. We characterised the NPs and assessed the viability (Alamar blue assay), formation of DNA strand breaks (mini-gel comet assay) and induction of micronucleus (MN) analysed using flow cytometry (in vitro microflow kit). The results confirmed the primary size (5 and 50 nm) but showed agglomeration of all NPs in the serum free medium used. Slight reduced cell viability (tested up to 50 mu g/ml) was observed following exposure to the Ag NPs of both particle sizes as well as to the smallest (5 nm) Au NPs. Similarly, at non-cytotoxic concentrations, both 5 and 50 nm-sized Ag NPs, as well as 5 nm-sized Au NPs, increased DNA strand breaks whereas for Pt NPs only the 50 nm size caused a slight increase in DNA damage. No clear induction of MN was observed in any of the doses tested (up to 20 mu g/ml). Taken together, by using the comet assay our study shows DNA strand breaks induced by Ag NPs, without any obvious differences in size, whereas effects from Au and Pt NPs were size-dependent in the sense that the 5 nm-sized Au NPs and 50 nm-sized Pt NPs particles were active. No clear induction of MN was observed for the NPs.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10603 - Genetics and heredity (medical genetics to be 3)

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

MUTAGENESIS

ISSN

0267-8357

e-ISSN

1464-3804

Volume of the periodical

33

Issue of the periodical within the volume

1

Country of publishing house

GB - UNITED KINGDOM

Number of pages

9

Pages from-to

77-85

UT code for WoS article

000426079100011

EID of the result in the Scopus database

2-s2.0-85042721477