Age determination in individual wild-caught Cimex lectularius using pteridine concentration
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F19%3A00107601" target="_blank" >RIV/00216224:14310/19:00107601 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Age determination in individual wild-caught Cimex lectularius using pteridine concentration
Original language description
The individual age for different insect species can be determined by the concentration of pteridines in the eye. The female age and with it the changing ability to keep viable sperm seems to be an important parameter in the study of ecological speciation and reproductive isolation of two host lines of bedbugs (C. lectularius) parasitizing humans and bats. No studies have yet been conducted to determine the age of the bed bug and there are still some methodological inconsistencies in determining the age we had to deal with, i.e. the selection of pteridine standards and extraction method, the presence of light during extraction, before we could proceed to compile a calibration curve for the dependence of pteridines on age. After optimizing the extraction and measurement of pteridine concentrations, we developed new methodological protocol. Of the five tested pteridine standards, Leucopterin showed the highest consistency within the age cohort and seems be most appropriate for determining the age of bedbugs in wild populations.
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
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OECD FORD branch
10613 - Zoology
Result continuities
Project
<a href="/en/project/GC18-08468J" target="_blank" >GC18-08468J: The role of sperm adaptation and sperm plasticity in ecological speciation</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů