Surface Display of Designer Protein Scaffolds on Genome-Reduced Strains of Pseudomonas putida
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F20%3A00114419" target="_blank" >RIV/00216224:14310/20:00114419 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1021/acssynbio.0c00276" target="_blank" >https://doi.org/10.1021/acssynbio.0c00276</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/acssynbio.0c00276" target="_blank" >10.1021/acssynbio.0c00276</a>
Alternative languages
Result language
angličtina
Original language name
Surface Display of Designer Protein Scaffolds on Genome-Reduced Strains of Pseudomonas putida
Original language description
The bacterium Pseudomonas putida KT2440 is gaining considerable interest as a microbial platform for biotechnological valorization of polymeric organic materials, such as lignocellulosic residues or plastics. However, P. putida on its own cannot make much use of such complex substrates, mainly because it lacks an efficient extracellular depolymerizing apparatus. We seek to address this limitation by adopting a recombinant cellulosome strategy for this host. In this work, we report an essential step in this endeavor—a display of designer enzyme-anchoring protein “scaffoldins”, encompassing cohesin binding domains from divergent cellulolytic bacterial species on the P. putida surface. Two P. putida chassis strains, EM42 and EM371, with streamlined genomes and differences in the composition of the outer membrane were employed in this study. Scaffoldin variants were optimally delivered to their surface with one of four tested autotransporter systems (Ag43 from Escherichia coli), and the efficient display was confirmed by extracellular attachment of chimeric beta-glucosidase and fluorescent proteins. Our results not only highlight the value of cell surface engineering for presentation of recombinant proteins on the envelope of Gram-negative bacteria but also pave the way toward designer cellulosome strategies tailored for P. putida.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
<a href="/en/project/GJ19-06511Y" target="_blank" >GJ19-06511Y: Orthogonalisation of carbohydrate metabolism in bacterial chassis Pseudomonas putida EM42 for co-utilisation of lignocellulose-derived sugars</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
ACS Synthetic Biology
ISSN
2161-5063
e-ISSN
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Volume of the periodical
9
Issue of the periodical within the volume
10
Country of publishing house
US - UNITED STATES
Number of pages
16
Pages from-to
2749-2764
UT code for WoS article
000582582100014
EID of the result in the Scopus database
2-s2.0-85093538854