Pseudomonas leptonychotis sp. nov., isolated from Weddell seals in Antarctica
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F20%3A00115510" target="_blank" >RIV/00216224:14310/20:00115510 - isvavai.cz</a>
Result on the web
<a href="https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003753" target="_blank" >https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003753</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1099/ijsem.0.003753" target="_blank" >10.1099/ijsem.0.003753</a>
Alternative languages
Result language
angličtina
Original language name
Pseudomonas leptonychotis sp. nov., isolated from Weddell seals in Antarctica
Original language description
A taxonomic study was carried out on four Gram-stain-negative strains P5773(T), P6169, P4708 and P6245, isolated from anus or mouth samples of Weddell seals at James Ross Island, Antarctica. The results of initial 16S rRNA gene sequence analysis showed that all four strains formed a group placed in the genus Pseudomonas and found Pseudomonas guineae and Pseudomonas peli to be their closest neighbours with 99.9 and 99.2% sequence similarity, respectively. Sequence analysis of rpoD, rpoB and gyrB housekeeping genes confirmed the highest similarity of isolates to P. peli (rpoD) and to P. guineae (rpoB and gyrB). The average nucleotide identity value below 86%, as calculated from the whole-genome sequence data, showed the low genomic relatedness of P5773(T) to its phylogenetic neighbours. The complete genome of strain P5773(T) was 4.4 Mb long and contained genes encoding proteins with biotechnological potential. The major fatty acids of the seal isolates were summed feature 8 (C-18:1 omega 7c), summed feature 3 (C-16 :1 omega 7c/C-16:1 omega 6c) and C-16:0. The major respiratory quinone was Q9. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. Putrescine and spermidine are predominant in the polyamine pattern. Further characterization performed using repetitive sequence-based PCR fingerprinting and MALDI-TOF MS analysis showed that the studied isolates formed a coherent cluster separated from the remaining Pseudomonas species and confirmed that they represent a novel species within the genus Pseudomonas, for which the name Pseudomonas leptonychotis sp. nov. is suggested. The type strain is P5773(T) (=CCM 8849(T)=LMG 30618(T)).
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
International Journal of systematic and Evolutionary Microbiology
ISSN
1466-5026
e-ISSN
1466-5034
Volume of the periodical
70
Issue of the periodical within the volume
1
Country of publishing house
GB - UNITED KINGDOM
Number of pages
7
Pages from-to
302-308
UT code for WoS article
000514419600043
EID of the result in the Scopus database
2-s2.0-85079021424