Pseudomonas petrae sp. nov. isolated from regolith samples in Antarctica
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26220%2F23%3APU148401" target="_blank" >RIV/00216305:26220/23:PU148401 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14310/23:00130840
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S0723202023000334" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0723202023000334</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.syapm.2023.126424" target="_blank" >10.1016/j.syapm.2023.126424</a>
Alternative languages
Result language
angličtina
Original language name
Pseudomonas petrae sp. nov. isolated from regolith samples in Antarctica
Original language description
A polyphasic taxonomic approach was used to characterize the four strains P2653T, P2652, P2498, and P2647, isolated from Antarctic regolith samples. Initial genotype screening performed by PCR fingerprinting based on repetitive sequences showed that the isolates studied formed a coherent cluster separated from the other Pseudomonas species. Identification results based on 16S rRNA gene sequences showed the highest sequence similarity with Pseudomonas graminis (99.7%), which was confirmed by multilocus sequence analysis using the rpoB, rpoD, and gyrB genes. Genome sequence comparison of P2653T with the most related P. graminis type strain DSM 11363T revealed an average nucleotide identity of 92.1% and a digital DNA-DNA hybridization value of 46.6%. The major fatty acids for all Antarctic strains were C16:0, Summed Feature 3 (C16:1 omega 7c/C16:1 omega 6c) and Summed Feature 8 (C18:1 omega 7c/C18:1 omega 6c). The predominant respiratory quinone was Q-9, and the major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, and phosphatidylglycerol. The regolith strains could be differentiated from related species by the absence of arginine dihydrolase, ornithine and lysine decarboxylase and by negative tyrosine hydrolysis. The results of this polyphasic study allowed the genotypic and phenotypic differentiation of four analysed strains from the closest related species, which confirmed that the strains represent a novel species within the genus Pseudomonas, for which the name Pseudomonas petrae sp. nov. is proposed with P2653T (CCM 8850T = DSM 112068T = LMG 30619T) as the type strain.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
20801 - Environmental biotechnology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
SYSTEMATIC AND APPLIED MICROBIOLOGY
ISSN
0723-2020
e-ISSN
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Volume of the periodical
46
Issue of the periodical within the volume
4
Country of publishing house
DE - GERMANY
Number of pages
9
Pages from-to
1-9
UT code for WoS article
001001607000001
EID of the result in the Scopus database
2-s2.0-85158014315