Comparison of 12 DNA extraction kits for vertebrate samples
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F20%3A00117063" target="_blank" >RIV/00216224:14310/20:00117063 - isvavai.cz</a>
Alternative codes found
RIV/00023272:_____/20:10134724 RIV/68081766:_____/20:00522567
Result on the web
<a href="https://doi.org/10.32800/abc.2020.43.0067" target="_blank" >https://doi.org/10.32800/abc.2020.43.0067</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.32800/abc.2020.43.0067" target="_blank" >10.32800/abc.2020.43.0067</a>
Alternative languages
Result language
angličtina
Original language name
Comparison of 12 DNA extraction kits for vertebrate samples
Original language description
Comparison of 12 DNA extraction kits for vertebrate samples. Obtaining high quality DNA extractions is a crucial step for molecular biology research projects. At present, numerous protocols are available for vertebrate tissue extractions. In the present study we compared eleven column-based protocols and one HotSHOT protocol using similar conditions (i.e., type of sample, weight of starting material). We evaluated time of extraction, quality and quantity of DNA yield, and price of extraction for a single sample. Based on our analysis, the most successful kits for producing DNA with the highest concentration and purity are the JetQuick (R) Genomic DNA Purification Kit (Genomed) and the NucleoSpin (R) Tissue (Macherey-Nagel). Nevertheless, it is highly recommended to test various extraction kits with specific samples to find the optimal kit in all aspects of time, quality and cost for a particular project.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10619 - Biodiversity conservation
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Animal Biodiversity and Conservation
ISSN
1578-665X
e-ISSN
2014-928X
Volume of the periodical
43
Issue of the periodical within the volume
1
Country of publishing house
ES - SPAIN
Number of pages
11
Pages from-to
67-77
UT code for WoS article
000536219200007
EID of the result in the Scopus database
2-s2.0-85079009639