The Quality of DNA Isolated from Processed Food and Feed via Different Extraction Procedures

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F19%3A43877352" target="_blank" >RIV/62157124:16270/19:43877352 - isvavai.cz</a>

Alternative codes found

RIV/00027162:_____/19:N0000015

Result on the web

<a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6471455/" target="_blank" >https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6471455/</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/molecules24061188" target="_blank" >10.3390/molecules24061188</a>

Result language

angličtina

Original language name

The Quality of DNA Isolated from Processed Food and Feed via Different Extraction Procedures

Original language description

The extraction of DNA is a critical step for species identification by PCR analysis in processed food and feed products. In this study, eight DNA extraction procedures were compared - DNeasy Blood and Tissue Kit, DNeasy mericon Food Kit, chemagic DNA Tissue 10 Kit, Food DNA Isolation Kit, UltraPrep Genomic DNA Food Mini Prep Kit, High Pure PCR Template Preparation Kit, phenolchloroform extraction, and NucleoSpin Food - using self-prepared samples from both raw and heat-processed and/or mechanically treated muscles and different types of meat products and pet food (pork, beef, and chicken). The yield, purity, and suitability of DNA for PCR amplification was evaluated. Additionally, comparisons between the effectiveness of various extraction methods were made with regard to price, and labor- and time-intensiveness. It was found that the DNeasy mericon Food Kit was the optimal choice for the extraction of DNA from raw muscle, heat-treated muscle, and homemade meat products from multiple and single species.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10608 - Biochemistry and molecular biology

Project

<a href="/en/project/QJ1530107" target="_blank" >QJ1530107: Methods for identification, traceability and authenticity verification of feed mixtures and food containing components of animal origin</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Molecules

ISSN

1420-3049

e-ISSN

—

Volume of the periodical

24

Issue of the periodical within the volume

6

Country of publishing house

CH - SWITZERLAND

Number of pages

10

Pages from-to

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UT code for WoS article

000465503800150

EID of the result in the Scopus database

2-s2.0-85063505887