The quality of DNA isolated from processed food and feed via different extraction procedures
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F19%3AN0000015" target="_blank" >RIV/00027162:_____/19:N0000015 - isvavai.cz</a>
Alternative codes found
RIV/62157124:16270/19:43877352
Result on the web
<a href="https://www.mdpi.com/1420-3049/24/6/1188" target="_blank" >https://www.mdpi.com/1420-3049/24/6/1188</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/molecules24061188" target="_blank" >10.3390/molecules24061188</a>
Alternative languages
Result language
angličtina
Original language name
The quality of DNA isolated from processed food and feed via different extraction procedures
Original language description
The extraction of DNA is a critical step for species identification by PCR analysis in processed food and feed products. In this study, eight DNA extraction procedures were compared: DNeasy Blood & Tissue Kit, DNeasy mericon Food Kit, chemagic DNA Tissue 10 Kit, Food DNA Isolation Kit, UltraPrep Genomic DNA Food Mini Prep Kit, High Pure PCR Template Preparation Kit, Phenol-chloroform extraction and NucleoSpin Food using self-prepared samples from both raw and heat-processed and/or mechanically treated muscles and different types of meat products and pet food (pork, beef and chicken). The yield, purity and suitability of DNA for PCR amplification was evaluated. Comparisons between the effectiveness of various kits were made with regard to price, labour- and time-intensiveness. It was found that the DNeasy mericon Food Kit was the optimal choice for the extraction of DNA from raw muscle, heat-treated muscle and home-made meat products from multiple and single species.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40401 - Agricultural biotechnology and food biotechnology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Molecules
ISSN
1420-3049
e-ISSN
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Volume of the periodical
24
Issue of the periodical within the volume
6
Country of publishing house
CH - SWITZERLAND
Number of pages
10
Pages from-to
1188
UT code for WoS article
000465503800150
EID of the result in the Scopus database
2-s2.0-85063505887