Assessment of DNA quality in processed tuna muscle tissues

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F16%3AN0000004" target="_blank" >RIV/00027162:_____/16:N0000004 - isvavai.cz</a>

Result on the web

<a href="http://www.potravinarstvo.com/cislo_casopisu/assessment-of-dna-quality-in-processed-tuna-muscle-tissues/" target="_blank" >http://www.potravinarstvo.com/cislo_casopisu/assessment-of-dna-quality-in-processed-tuna-muscle-tissues/</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.5219/612" target="_blank" >10.5219/612</a>

Result language

angličtina

Original language name

Assessment of DNA quality in processed tuna muscle tissues

Original language description

The quality of species specific DNA crucially affects the efficiency of amplification during the subsequent PCR. The problem in DNA detection in canned products lies in the possibility of the fragmentation of DNA during the processing technologies and the use of ingredients (oil, salt, spice), that may inhibit the PCR reaction. In this study three DNA extraction methods were compared: DNeasy Blood and Tissue Kit, DNeasy mericon Food Kit and Chemagic DNA tissue 10 Kit. The quantity and quality of DNA were evaluated by measuring DNA concentration and ratios A260/A280. Several parameters were estimated: the effect of whole and mechanically treated muscle, sterilization procedure used in canned process (high temperature in combination with high pressure) and addition of raw materials. The highest DNA concentrations were observed in non-processed muscle that is not influenced by the sterilization process. DNeasy mericon Food Kit produced DNA of higher concentration in non-processed sample, Chemagic DNA tissue 10 Kit delivered higher DNA yields than kits DNeasy Blood and Tissue Kit and DNeasy mericon Food Kit in canned samples, although the purity was lower, but still within the range 1.7 – 2.0. DNA was considered to be satisfactorily pure in all three types of samples and using all three types of DNA isolation. In case of the samples enriched of ingredients and treated with sterilization process as whole or ground muscle Chemagic DNA tissue 10 Kit produced in all samples (whole and ground muscle) the highest values of DNA concentration, but almost all values of A260/A280 were lower than 1.7. Therefore DNeasy mericon Food Kit appears to be a favorite one, in all samples with whole muscle gives higher values of DNA concentrations than DNeasy Blood and Tissue Kit. The impact of ingredients was not conclusively approved and their importance to the suitability of extracted DNA for PCR amplification is needed to be discussed in further analysis.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GM - Food industry

OECD FORD branch

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Project

<a href="/en/project/QJ1530107" target="_blank" >QJ1530107: Methods for identification, traceability and authenticity verification of feed mixtures and food containing components of animal origin</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Potravinárstvo

ISSN

1337-0960

e-ISSN

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Volume of the periodical

10

Issue of the periodical within the volume

1

Country of publishing house

SK - SLOVAKIA

Number of pages

8

Pages from-to

308-315

UT code for WoS article

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EID of the result in the Scopus database

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