Making good’s buffers good for freezing: The acidity changes and their elimination via mixing with sodium phosphate

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F21%3A00118798" target="_blank" >RIV/00216224:14310/21:00118798 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1016/j.ijpharm.2020.120128" target="_blank" >https://doi.org/10.1016/j.ijpharm.2020.120128</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.ijpharm.2020.120128" target="_blank" >10.1016/j.ijpharm.2020.120128</a>

Result language
angličtina
Original language name
Making good’s buffers good for freezing: The acidity changes and their elimination via mixing with sodium phosphate
Original language description
Solutions of three Good’s buffers (HEPES, MOPS, and MES), both pure and mixed with sodium phosphate buffers (Na-P), are investigated in terms of the freezing-induced acidity changes in their operational pH ranges. The Good’s buffers have the tendency to basify upon freezing and, more intensively, at lower pHs. The acidity varies most prominently in MES, where the change may reach the value of two. Importantly, the Good’s buffers are shown to mitigate the strong acidification in the Na-P buffer. Diverse concentrations of the Good’s buffers are added to cancel out the strong, freezing-induced acidity drop in 50 mM Na-P that markedly contributes to the solution’s acidity; the relevant values are 3 mM HEPES, 10 mM MOPS, and 80 mM MES. These buffer blends are therefore proposed to be applied in maintaining approximately the acidity of solutions even after the freezing process and, as such, should limit the stresses for frozen chemicals and biochemicals.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10403 - Physical chemistry

Project
<a href="/en/project/GA19-08239S" target="_blank" >GA19-08239S: The Spectroscopy and Microscopy of Chemical Compounds in Ice within the Environmental and Pharmaceutical Domains</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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