Effect of buffer in simulated body fluid on morphology and crystallinity of hydroxyapatite precipitated on 45S5 bioactive glass-derived glass-ceramic scaffolds: comparison of Good´s buffer system and tris

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22310%2F21%3A43922237" target="_blank" >RIV/60461373:22310/21:43922237 - isvavai.cz</a>

Result on the web

<a href="https://www.sciencedirect.com/science/article/pii/S2468519421001075" target="_blank" >https://www.sciencedirect.com/science/article/pii/S2468519421001075</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.mtchem.2021.100527" target="_blank" >10.1016/j.mtchem.2021.100527</a>

Result language

angličtina

Original language name

Effect of buffer in simulated body fluid on morphology and crystallinity of hydroxyapatite precipitated on 45S5 bioactive glass-derived glass-ceramic scaffolds: comparison of Good´s buffer system and tris

Original language description

In vitro tests that verify the ability of a material to form bone-like apatite (HAp) use a solution that imitates the inorganic part of blood plasma, simulated body fluid (SBF). During such tests, it is necessary to maintain a neutral pH, for which purpose the International Standards Organization recommends the TRIS buffer (ISO - 23317:2014). To do this, TRIS buffer must remain inert, but, as we have previously reported, TRIS interacts with highly bioreactive materials (such as 45S5 Bioglass derived scaffolds), thereby accelerating scaffold dissolution. In the search for an alternative to TRIS, we have also recently published results for the HEPES and MOPS buffers, which were also unable to maintain a neutral pH in SBF. Thus, we here continue our search for a more suitable Good´s buffer by comparing BES (N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid) and TES (2-[(2-Hydroxy-1,1-bis(hydroxymethyl) ethyl) amino] ethanesulfonic acid) with TRIS. 45S5-derived glass-ceramic scaffolds were tested under static-dynamic conditions for bone-like apatite formation using SBF+BES and SBF+TES solutions. The pH measurements, leachate analysis [AAS (Ca2+, SiIV), UV-VIS spectrophotometry (PO4)3-] and scaffold analysis (XRD, XRF, SEM/EDS, BET) all showed that both BES and TES quickly interacted with the tested material. Moreover, the kinetics of the glass-ceramic dissolution affected the crystallinity and morphology of the precipitated HAp. Based on our previous results and the present data, it appears that Good´s buffers are not suitable for the in vitro testing of bioactivity of highly bioreactive materials.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

20504 - Ceramics

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Materials Today Chemistry

ISSN

2468-5194

e-ISSN

—

Volume of the periodical

21

Issue of the periodical within the volume

Neuveden

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

9

Pages from-to

1-9

UT code for WoS article

000696937300014

EID of the result in the Scopus database

2-s2.0-85111318825