Effect of buffer in simulated body fluid on morphology and crystallinity of hydroxyapatite precipitated on 45S5 bioactive glass-derived glass–ceramic scaffolds: comparison of Good's buffer systems and TRIS
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388980%3A_____%2F21%3A00544522" target="_blank" >RIV/61388980:_____/21:00544522 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1016/j.mtchem.2021.100527" target="_blank" >https://doi.org/10.1016/j.mtchem.2021.100527</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.mtchem.2021.100527" target="_blank" >10.1016/j.mtchem.2021.100527</a>
Alternative languages
Result language
angličtina
Original language name
Effect of buffer in simulated body fluid on morphology and crystallinity of hydroxyapatite precipitated on 45S5 bioactive glass-derived glass–ceramic scaffolds: comparison of Good's buffer systems and TRIS
Original language description
In vitro tests that verify the ability of a material to form bone-like apatite precipitated (HAp) use a solution that imitates the inorganic part of blood plasma, simulated body fluid (SBF). During such tests, it is necessary to maintain a neutral pH, for which purpose the International Standards Organization recommends the TRIS (tris(hydroxymethyl)aminomethane) buffer (ISO 23,317:2014). To do this, TRIS buffer must remain inert, but, as we have previously reported, TRIS interacts with highly bioreactive materials (such as 45S5 Bioglass-derived scaffolds), thereby accelerating scaffold dissolution. In the search for an alternative to TRIS, we have also recently published results for the 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) and 3-(N-morpholino) propanesulfonic acid (MOPS) buffers, which were also unable to maintain a neutral pH in SBF. Thus, we here continue our search for a more suitable Good's buffer by comparing BES (N,N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid) and TES (2-[(2-hydroxy-1,1-bis(hydroxymethyl) ethyl) amino] ethanesulfonic acid) with TRIS. 45S5-derived glass–ceramic scaffolds were tested under static–dynamic conditions for bone-like apatite formation using SBF + BES and SBF + TES solutions. The pH measurements, leachate analysis [atomic absorption spectrophotometry (AAS) (Ca2+, SiIV), UltraViolet-Visible (UV-VIS) spectrophotometry (PO4)3−], and scaffold analysis [X-Ray powder diffraction analysis (XRD), X-ray fluorescent analysis (XRF), scanning electron microscopy (SEM)/energy dispersive spectroscopy (EDS), Brunauer.Emmett.Teller specific surface area analysis (BET)] all showed that both BES and TES quickly interacted with the tested material. Moreover, the kinetics of the glass–ceramic dissolution affected the crystallinity and morphology of the precipitated HAp. Based on our previous results and the present data, it appears that Good's buffers are not suitable for the in vitro testing of bioactivity of highly bioreactive materials.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10402 - Inorganic and nuclear chemistry
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Materials Today Chemistry
ISSN
2468-5194
e-ISSN
2468-5194
Volume of the periodical
21
Issue of the periodical within the volume
AUG
Country of publishing house
GB - UNITED KINGDOM
Number of pages
9
Pages from-to
100527
UT code for WoS article
000696937300014
EID of the result in the Scopus database
2-s2.0-85111318825