Bioconjugates of photon-upconversion nanoparticles for cancer biomarker detection and imaging
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F22%3A00119663" target="_blank" >RIV/00216224:14310/22:00119663 - isvavai.cz</a>
Alternative codes found
RIV/68081715:_____/22:00554418 RIV/61389013:_____/22:00554418
Result on the web
<a href="https://www.nature.com/articles/s41596-021-00670-7" target="_blank" >https://www.nature.com/articles/s41596-021-00670-7</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s41596-021-00670-7" target="_blank" >10.1038/s41596-021-00670-7</a>
Alternative languages
Result language
angličtina
Original language name
Bioconjugates of photon-upconversion nanoparticles for cancer biomarker detection and imaging
Original language description
The detection of cancer biomarkers in histological samples and blood is of paramount importance for clinical diagnosis. Current methods are limited in terms of sensitivity, hindering early detection of disease. We have overcome the shortcomings of currently available staining and fluorescence labeling methods by taking an integrative approach to establish photon-upconversion nanoparticles (UCNP) as a powerful platform for cancer detection. These nanoparticles are readily synthesized in different sizes to yield efficient and tunable short-wavelength light emission under near-infrared excitation, which eliminates optical background interference of the specimen. Here we present a protocol for the synthesis of UCNPs by high-temperature co-precipitation or seed-mediated growth by thermal decomposition, surface modification by silica or poly(ethylene glycol) that renders the particles resistant to nonspecific binding, and the conjugation of streptavidin or antibodies for biological detection. To detect blood-based biomarkers, we present an upconversion-linked immunosorbent assay for the analog and digital detection of the cancer marker prostate-specific antigen. When applied to immunocytochemistry analysis, UCNPs enable the detection of the breast cancer marker human epidermal growth factor receptor 2 with a signal-to-background ratio 50-fold higher than conventional fluorescent labels. UCNP synthesis takes 4.5 d, the preparation of the antibody–silica–UCNP conjugate takes 3 d, the streptavidin–poly(ethylene glycol)–UCNP conjugate takes 2–3 weeks, upconversion-linked immunosorbent assay takes 2–4 d and immunocytochemistry takes 8–10 h. The procedures can be performed after standard laboratory training in nanomaterials research.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Nature Protocols
ISSN
1754-2189
e-ISSN
1750-2799
Volume of the periodical
17
Issue of the periodical within the volume
April 2022
Country of publishing house
DE - GERMANY
Number of pages
47
Pages from-to
1028-1072
UT code for WoS article
000757750800001
EID of the result in the Scopus database
2-s2.0-85124960324