Proteomic Signatures of Human Visceral and Subcutaneous Adipocytes
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F22%3A00125133" target="_blank" >RIV/00216224:14310/22:00125133 - isvavai.cz</a>
Alternative codes found
RIV/61988987:17450/22:A2302E3T
Result on the web
<a href="https://academic.oup.com/jcem/article/107/3/755/6406610" target="_blank" >https://academic.oup.com/jcem/article/107/3/755/6406610</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1210/clinem/dgab756" target="_blank" >10.1210/clinem/dgab756</a>
Alternative languages
Result language
angličtina
Original language name
Proteomic Signatures of Human Visceral and Subcutaneous Adipocytes
Original language description
Context: Adipose tissue distribution is a key factor influencing metabolic health and risk in obesity-associated comorbidities. Objective: Here we aim to compare the proteomic profiles of mature adipocytes from different depots. Methods: Abdominal subcutaneous (SA) and omental visceral adipocytes (VA) were isolated from paired adipose tissue biopsies obtained during bariatric surgery on 19 severely obese women (body mass index > 30 kg/m(2)) and analyzed using state-of-the-art mass spectrometry. Differential expression analysis and weighted gene co-expression network analysis (WGCNA) were performed to investigate proteome signature properties and to examine a possible association of the protein expression with the clinical data. Results: We identified 3686 protein groups and found 1140 differentially expressed proteins (adj. P value < 0.05), of which 576 proteins were upregulated in SA and 564 in VA samples. We provide a global protein profile of abdominal SA and omental VA, present the most differentially expressed pathways and processes distinguishing SA from VA, and correlate them with clinical and body composition data. We show that SA are significantly more active in processes linked to vesicular transport and secretion, and to increased lipid metabolism activity. Conversely, the expression of proteins involved in the mitochondria! energy metabolism and translational or biosynthetic activity is higher in VA. Conclusion: Our analysis represents a valuable resource of protein expression profiles in abdominal SA and omental VA, highlighting key differences in their role in obesity.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30202 - Endocrinology and metabolism (including diabetes, hormones)
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Clinical Endocrinology and Metabolism
ISSN
0021-972X
e-ISSN
1945-7197
Volume of the periodical
107
Issue of the periodical within the volume
3
Country of publishing house
GB - UNITED KINGDOM
Number of pages
21
Pages from-to
755-775
UT code for WoS article
000756897900013
EID of the result in the Scopus database
2-s2.0-85124850550