Impedimetric immunosensor for microalbuminuria based on a WS2/Au water-phase assembled nanocomposite
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F23%3A00133461" target="_blank" >RIV/00216224:14310/23:00133461 - isvavai.cz</a>
Result on the web
<a href="https://link.springer.com/article/10.1007/s00604-023-05873-1" target="_blank" >https://link.springer.com/article/10.1007/s00604-023-05873-1</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s00604-023-05873-1" target="_blank" >10.1007/s00604-023-05873-1</a>
Alternative languages
Result language
angličtina
Original language name
Impedimetric immunosensor for microalbuminuria based on a WS2/Au water-phase assembled nanocomposite
Original language description
An electrochemical impedimetric biosensor for human serum albumin (HSA) determination is proposed. The biosensor is based on water-phase assembled nanocomposites made of 2D WS2 nanoflakes and Au nanoparticles (AuNPs). The WS2 has been produced using a liquid-phase exfoliation strategy assisted by sodium cholate, obtaining a water-stable suspension that allowed the straightforward decoration with AuNPs directly in the aqueous phase. The resulting WS2/Au nanocomposite has been characterized by atomic force microscopy and Raman spectroscopy and, then, employed to modify screen-printed electrodes. Good electron-transfer features have been achieved. An electrochemical immunosensing platform has been assembled exploiting cysteamine- glutaraldehyde covalent chemistry for antibody (Ab) immobilization. The resulting immunosensor exhibited good sensitivity for HSA detection (LOD = 2 ng mL(-1)), with extended linear range (0.005 - 100 mu g mL(-1)), providing a useful analytical tool for HSA determination in urine at relevant clinical ranges for microalbuminuria screening. The HSA quantification in human urine samples resulted in recoveries from 91.8 to 112.4% and was also reproducible (RSD < 7.5%, n = 3), with marked selectivity. This nanocomposite, thanks to the reliable performance and the ease of the assembling strategy, is a promising alternative for electrochemical immunosensing of health relevant markers.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
<a href="/en/project/LM2023042" target="_blank" >LM2023042: Czech Infrastructure for Integrative Structural Biology</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Microchimica Acta
ISSN
0026-3672
e-ISSN
1436-5073
Volume of the periodical
190
Issue of the periodical within the volume
8
Country of publishing house
AT - AUSTRIA
Number of pages
12
Pages from-to
1-12
UT code for WoS article
001033463400005
EID of the result in the Scopus database
2-s2.0-85165306747