A simple electrochemical biosensor for the detection of methylated DNA and for methyltransferase activity monitoring

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F19%3APU134921" target="_blank" >RIV/00216305:26620/19:PU134921 - isvavai.cz</a>
Alternative codes found
RIV/62156489:43210/19:43917138
Result on the web
<a href="https://mendelnet.cz/artkey/mnt-201901-0120_A-simple-electrochemical-biosensor-for-the-detection-of-methylated-DNA-and-for-methyltransferase-activity-monit.php?back=/magno/mnt/2019/mn1.php?secid=4" target="_blank" >https://mendelnet.cz/artkey/mnt-201901-0120_A-simple-electrochemical-biosensor-for-the-detection-of-methylated-DNA-and-for-methyltransferase-activity-monit.php?back=/magno/mnt/2019/mn1.php?secid=4</a>
DOI - Digital Object Identifier
—

Result language
angličtina
Original language name
A simple electrochemical biosensor for the detection of methylated DNA and for methyltransferase activity monitoring
Original language description
DNA methylation is one of the well-known epigenetic mechanism which plays a crucial role in the development of various diseases, such as cancer, cardiovascular diseases or diabetes. Electrochemical biosensors promise excellent results for clinical diagnostics, especially in term of their sensitivity, stability, selectivity, portability, they are cost-effective, easy-to-use and provides a fast response. In this study, simply, reliably and selective DNA based electrochemical biosensor for detection of methylated DNA was fabricated. The proposed biosensor was modified with the synthesised reduced graphene oxide combined with gold nanoparticles (rGO-AuNPs). This nanocomposite has shown a strong affinity to the DNA probe and demonstrated promising analytical characteristics. The electrochemical impedance spectroscopy (EIS) was used for the characterization of interface properties of the gold electrode (GE). Additionally, the sensitivity of the developed biosensor was performed by differential pulse voltammetry (DPV) to investigate the activity of enzyme methyltransferase M.SssI (MTase). Fabricated biosensor offers quite a low detection limit (LOD), which was 3.2 U/ml and limit of quantification (LOQ) was 3.3 U/ml.
Czech name
—
Czech description
—

Project
<a href="/en/project/LQ1601" target="_blank" >LQ1601: CEITEC 2020</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
MendelNet 2019 Proceedings of 26th International PhD Students Conference
ISBN
978-80-7509-688-3
ISSN
—
e-ISSN
—
Number of pages
6
Pages from-to
621-626
Publisher name
Mendel University in Brno
Place of publication
Neuveden
Event location
Brno
Event date
Nov 6, 2019
Type of event by nationality
CST - Celostátní akce
UT code for WoS article
—

Similar results(10)