Leptospira interrogans outer membrane protein-based nanohybrid sensor for the diagnosis of leptospirosis

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62690094%3A18450%2F21%3A50017989" target="_blank" >RIV/62690094:18450/21:50017989 - isvavai.cz</a>

Result on the web

<a href="https://www.mdpi.com/1424-8220/21/7/2552" target="_blank" >https://www.mdpi.com/1424-8220/21/7/2552</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/s21072552" target="_blank" >10.3390/s21072552</a>

Result language

angličtina

Original language name

Leptospira interrogans outer membrane protein-based nanohybrid sensor for the diagnosis of leptospirosis

Original language description

Leptospirosis is an underestimated tropical disease caused by the pathogenic Leptospira species and responsible for several serious health problems. Here, we aimed to develop an ultrasen-sitive DNA biosensor for the rapid and on-site detection of the Loa22 gene of Leptospira interrogans using a gold nanoparticle–carbon nanofiber composite (AuN/CNF)-based screen-printed electrode. Cyclic voltammetry and electrochemical impedance were performed for electrochemical analysis. The sensitivity of the sensor was 5431.74 µA/cm2 /ng with a LOD (detection limit) of 0.0077 ng/µL using cyclic voltammetry. The developed DNA biosensor was found highly specific to the Loa22 gene of L. interrogans, with a storage stability at 4◦ C for 180 days and a 6% loss of the initial response. This DNA-based sensor only takes 30 min for rapid detection of the pathogen, with a higher specificity and sensitivity. The promising results obtained suggest the application of the developed sensor as a point of care device for the diagnosis of leptospirosis. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10406 - Analytical chemistry

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Sensors

ISSN

1424-8220

e-ISSN

—

Volume of the periodical

21

Issue of the periodical within the volume

7

Country of publishing house

CH - SWITZERLAND

Number of pages

10

Pages from-to

"Article number 2552"

UT code for WoS article

000638865700001

EID of the result in the Scopus database

2-s2.0-85103570606