Lighting up tetracyclines: Heavy water solvent effects on antibiotic fluorescence
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F25%3A00142583" target="_blank" >RIV/00216224:14310/25:00142583 - isvavai.cz</a>
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S0026265X25030851" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0026265X25030851</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.microc.2025.115737" target="_blank" >10.1016/j.microc.2025.115737</a>
Alternative languages
Result language
angličtina
Original language name
Lighting up tetracyclines: Heavy water solvent effects on antibiotic fluorescence
Original language description
The fluorescence properties of tetracycline antibiotics (tetracycline, minocycline, doxycycline, and omadacycline) were investigated in water and heavy water to evaluate the influence of solvent effects and the presence of metal ions on their spectral behavior. Emission spectra were recorded, both in the absence and presence of divalent metal cations (Mg2+, Ca2+) and the monovalent Li+ ion. All compounds exhibited enhanced fluorescence in D2O compared to H2O, with a marked increase upon the addition of Mg2+ ions. Emission maxima shifted in the presence of Mg2+, and fluorescence intensities reached up to 34.5-fold higher for omadacycline in D2O with Mg2+. Li+ showed no significant effect due to its weak coordination properties; however, fluorescence enhancement was still observed in D2O, indicating the sole influence of solvent even in the absence of strong complex formation. Chromatographic separation was successfully achieved for tetracycline, minocycline, and omadacycline using HPLC coupled with fluorescence detection. The combined use of D2O and Mg2+ not only improved detection sensitivity but also lowered limits of detection (LODs), reaching as low as 3.1 · 10−9 mol·L−1 for omadacycline. These findings highlight the cumulative effects of solvent and metal ion environments on tetracycline fluorescence and provide valuable insights for developing sensitive analytical methods for antibiotic detection in pharmaceutical, clinical, and environmental samples.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Microchemical Journal
ISSN
0026-265X
e-ISSN
1095-9149
Volume of the periodical
218
Issue of the periodical within the volume
November
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
7
Pages from-to
115737
UT code for WoS article
001603459600004
EID of the result in the Scopus database
2-s2.0-105018866276