Uukuniemi virus infection causes a pervasive remodelling of the RNA-binding proteome in tick cells

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F25%3A00143279" target="_blank" >RIV/00216224:14310/25:00143279 - isvavai.cz</a>

Result on the web

<a href="https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1013393" target="_blank" >https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1013393</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1371/journal.ppat.1013393" target="_blank" >10.1371/journal.ppat.1013393</a>

Result language

angličtina

Original language name

Uukuniemi virus infection causes a pervasive remodelling of the RNA-binding proteome in tick cells

Original language description

Cellular RNA-binding proteins (RBPs) are pivotal for the viral lifecycle, mediating key host-virus interactions that promote or repress virus infection. While these interactions have been largely studied in the vertebrate host, no comprehensive analyses of protein-RNA interactions occurring in cells of arbovirus vectors, in particular ticks, have been performed to date. Here we systematically identified the responses of the RNA-binding proteome (RBPome) to infection with a prototype bunyavirus (Uukuniemi virus; UUKV) in tick cells and discovered changes in RNA-binding activity for 283 proteins. In an orthogonal approach, we analysed the composition of the viral ribonucleoprotein by immunoprecipitation of UUKV nucleocapsid protein (N) in infected cells. We found many tick RBPs that are regulated by UUKV infection and associate with viral nucleocapsid protein complexes, and we confirmed experimentally that they impact UUKV infection. This includes the tick homolog of topoisomerase 3B (TOP3B), a protein able to manipulate the topology of RNA, which particularly affected viral particle production. Our data thus reveals the first protein-RNA interaction map for infected tick cells.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10607 - Virology

Project

—

Continuities

S - Specificky vyzkum na vysokych skolach

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

PLOS PATHOGENS

ISSN

1553-7366

e-ISSN

1553-7374

Volume of the periodical

21

Issue of the periodical within the volume

8

Country of publishing house

US - UNITED STATES

Number of pages

26

Pages from-to

1-26

UT code for WoS article

001554702700005

EID of the result in the Scopus database

2-s2.0-105012270558