Tick salivary cystatin Iristatin limits the virus replication in skin of tick-borne encephalitis virus-infected mice

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F25%3A00605149" target="_blank" >RIV/60077344:_____/25:00605149 - isvavai.cz</a>

Alternative codes found

RIV/60076658:12310/25:43909909 RIV/00027162:_____/25:N0000009

Result on the web

<a href="https://doi.org/10.1007/s00436-024-08441-5" target="_blank" >https://doi.org/10.1007/s00436-024-08441-5</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00436-024-08441-5" target="_blank" >10.1007/s00436-024-08441-5</a>

Result language

angličtina

Original language name

Tick salivary cystatin Iristatin limits the virus replication in skin of tick-borne encephalitis virus-infected mice

Original language description

Tick-borne encephalitis virus (TBEV) is flavivirus transmitted to the host via tick saliva which contains various molecules with biological impacts. One of such molecules is Iristatin, a cysteine protease inhibitor from Ixodes ricinus that has been shown to have immunomodulatory properties. To characterize Iristatin in the relation to TBEV, we investigate whether this tick inhibitor has any capacity to influence TBEV infection. Mice were intradermally infected by TBEV with or without Iristatin and the viral multiplication was determined in skin and brain tissues by RT-PCR two and 5 days after infection. The viral RNA was detected in both intervals in skin and increased by time. The application of Iristatin caused a reduction in viral RNA in skin but not in the brain of infected mice 5 days post-infection. Moreover, anti-viral effect of Iristatin on skin was accompanied by a significant decline of interferon-stimulated gene 15 gene expression. The effect of Iristatin on TBEV replication was tested also in vitro in primary macrophages and dendritic cells, however, no changes were observed suggesting no direct interference of Iristatin with virus replication. Still, the Iristatin caused a suppression of Erk1/2 phosphorylation in TBEV-infected dendritic cells and had the anti-apoptotic effect. This is the first report showing that a tick cystatin decreases the viral RNA in the host skin, likely indirectly through creating skin environment that is less supportive for TBEV replication. Assuming, that viral RNA reflects the amount of infectious virus, decline of TBEV in host skin could influence the tick biology or virus transmission during cofeeding.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10607 - Virology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Parasitology Research

ISSN

0932-0113

e-ISSN

1432-1955

Volume of the periodical

124

Issue of the periodical within the volume

1

Country of publishing house

DE - GERMANY

Number of pages

10

Pages from-to

8

UT code for WoS article

001401231600002

EID of the result in the Scopus database

2-s2.0-85216050225