Type of Extender and Equilibration Time as Factors Affecting Post-Thaw Quality Characteristics of Turkey Semen
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F25%3A00143381" target="_blank" >RIV/00216224:14310/25:00143381 - isvavai.cz</a>
Result on the web
<a href="https://www.mdpi.com/2076-2615/15/21/3218" target="_blank" >https://www.mdpi.com/2076-2615/15/21/3218</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/ani15213218" target="_blank" >10.3390/ani15213218</a>
Alternative languages
Result language
angličtina
Original language name
Type of Extender and Equilibration Time as Factors Affecting Post-Thaw Quality Characteristics of Turkey Semen
Original language description
Commercial turkey breeding relies almost entirely on artificial insemination, yet avian sperm are unusually vulnerable to cooling and freezing injury. As a result, extender chemistry and processing steps, especially low-temperature equilibration, are pivotal for post-thaw performance. We evaluated how extender choice, paired with equilibration time, shapes the post-thaw quality of turkey semen. Ejaculates were diluted in Beltsville, Sperm Motility Medium (SMM), Botucrio, or Kobidil(+), then equilibrated for 20 or 40 min before freezing; samples were cryostored for one month and assessed immediately after thawing. The outcomes included motility/kinematics, membrane integrity, mitochondrial activity and membrane potential, apoptosis/necrosis, reactive oxygen species (ROS), DNA fragmentation, and bacteriological load. Overall, 20 min equilibration improved post-thaw motility and membrane integrity, and reduced DNA fragmentation and ROS. Among extenders, Beltsville delivered the best overall sperm quality. Considering the extender x time interaction, Beltsville, Botucrio, and Kobidil(+) performed best at 20 min, whereas SMM performed best at 40 min. Thus, Beltsville and SMM provide strong, time-specific options for turkey semen cryopreservation-Beltsville at 20 min and SMM at 40 min.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40301 - Veterinary science
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Animals
ISSN
2076-2615
e-ISSN
2076-2615
Volume of the periodical
15
Issue of the periodical within the volume
21
Country of publishing house
CH - SWITZERLAND
Number of pages
15
Pages from-to
1-15
UT code for WoS article
001616714100001
EID of the result in the Scopus database
2-s2.0-105021431530