Multicolor Upconversion Forster Resonant Energy Transfer Using Optimized Yb@YbTm Core@Shell Nanoparticles
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F25%3A00144628" target="_blank" >RIV/00216224:14310/25:00144628 - isvavai.cz</a>
Result on the web
<a href="https://pubs.acs.org/doi/10.1021/acsnano.5c13869" target="_blank" >https://pubs.acs.org/doi/10.1021/acsnano.5c13869</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/acsnano.5c13869" target="_blank" >10.1021/acsnano.5c13869</a>
Alternative languages
Result language
angličtina
Original language name
Multicolor Upconversion Forster Resonant Energy Transfer Using Optimized Yb@YbTm Core@Shell Nanoparticles
Original language description
Upconverting nanoparticles (UCNPs) have emerged as promising alternative donors for resonance energy transfer (FRET)-based biosensing. However, employing UCNPs in FRET assays remains challenging because they display relatively small absorption cross sections and are relatively large as compared to the Forster distance. Thousands of individual donor ions in each UCNP are located within various distances from surface-bound acceptors, complicating the data analysis. While previous studies have explored how the composition and architecture of UCNPs influence FRET, many reports remain qualitative, and multicolor UC-FRET systems involving a single donor and multiple acceptors are less commonly studied than single-donor-single-acceptor systems. To address these challenges, we synthesized UCNPs with an absorbing core (Yb3+-doped)/active shell (Yb3+, Tm3+-doped) nanoparticles systematically varying Tm3+ concentrations to optimize the FRET efficiency to surface-bound organic acceptors. A shell composition containing 4% Tm3+ yielded the highest FRET efficiency. Moreover, four distinct ATTO dyes showing spectral overlap with the blue emission of Tm3+ were used as acceptor dyes on the surface of UCNPs to evaluate FRET efficiencies in spectral and time domains. The differentiation of the four ATTO dyes on one type of upconverting donor nanoparticles using a simple ratiometric approach lays the foundation for the design of multiplexed bioassays. Our results offer a strategy for improving UC-FRET sensitivity through smart core-shell UCNPs designs, donor concentration tuning, and provide important insights into the rational design of more efficient, multicolor, and wash-free UC biosensing platforms.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30403 - Technologies involving identifying the functioning of DNA, proteins and enzymes and how they influence the onset of disease and maintenance of well-being (gene-based diagnostics and therapeutic interventions [pharmacogenomics, gene-based therapeutics])
Result continuities
Project
<a href="/en/project/GF23-06199K" target="_blank" >GF23-06199K: Novel anti-Stokes lanthanide nanoparticles and multicolor FRET mechanisms for single-molecule DNA sequencing</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
ACS NANO
ISSN
1936-0851
e-ISSN
1936-086X
Volume of the periodical
19
Issue of the periodical within the volume
48
Country of publishing house
US - UNITED STATES
Number of pages
11
Pages from-to
41110-41120
UT code for WoS article
001623202000001
EID of the result in the Scopus database
2-s2.0-105024260337