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Properties of RNase immobilized on magnetic particles

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F99%3A00004114" target="_blank" >RIV/00216224:14310/99:00004114 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Properties of RNase immobilized on magnetic particles

  • Original language description

    In this study, RNase A immobilized on magnetic supports based on bead cellulose differeing in particle size and poly(HEMA-co-EDMA)microsheres were used. Enzyme was coupled to particles via cyanuric chloride method. Thermal stability of soluble and immobilzed RNase a was tested. Soluble and immobilized enzymes were preincubated in water, acetate buffer (pH 5.0) and tsic-NaCl buffer (pH 7.5) for 1 h. at different temperatures. The activity of enzyme was estimated fro UV spectrophometrical measurement. Thematrices base on magnetic bead cellulose and magnetic poly(HEMA-co-EDMA) have a low tendency to adsorb RNA.

  • Czech name

  • Czech description

Classification

  • Type

    D - Article in proceedings

  • CEP classification

    EI - Biotechnology and bionics

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/GA203%2F98%2F1231" target="_blank" >GA203/98/1231: Oriented immobilization of enzymes and antibodies using biospecific interactions</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    1999

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Article name in the collection

    13th Bratislava International Conference on Polymers, Separation and Characterization of Macromolecules

  • ISBN

  • ISSN

  • e-ISSN

  • Number of pages

    1

  • Pages from-to

    144

  • Publisher name

    Polymer Institute of the Slovak Academy of Sciences

  • Place of publication

    Bratislava

  • Event location

    Bratislava

  • Event date

    Jan 1, 1999

  • Type of event by nationality

    WRD - Celosvětová akce

  • UT code for WoS article