Properties of RNase immobilized on magnetic particles
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F99%3A00004114" target="_blank" >RIV/00216224:14310/99:00004114 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Properties of RNase immobilized on magnetic particles
Original language description
In this study, RNase A immobilized on magnetic supports based on bead cellulose differeing in particle size and poly(HEMA-co-EDMA)microsheres were used. Enzyme was coupled to particles via cyanuric chloride method. Thermal stability of soluble and immobilzed RNase a was tested. Soluble and immobilized enzymes were preincubated in water, acetate buffer (pH 5.0) and tsic-NaCl buffer (pH 7.5) for 1 h. at different temperatures. The activity of enzyme was estimated fro UV spectrophometrical measurement. Thematrices base on magnetic bead cellulose and magnetic poly(HEMA-co-EDMA) have a low tendency to adsorb RNA.
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
EI - Biotechnology and bionics
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GA203%2F98%2F1231" target="_blank" >GA203/98/1231: Oriented immobilization of enzymes and antibodies using biospecific interactions</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
1999
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
13th Bratislava International Conference on Polymers, Separation and Characterization of Macromolecules
ISBN
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ISSN
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e-ISSN
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Number of pages
1
Pages from-to
144
Publisher name
Polymer Institute of the Slovak Academy of Sciences
Place of publication
Bratislava
Event location
Bratislava
Event date
Jan 1, 1999
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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