Characterisation of Ribonuclease A Immobilized on Magnetic Cellulose - and Poly(2-Hydroxyethyl Methacrylate)-Based Supports

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F00%3A00003595" target="_blank" >RIV/00216224:14310/00:00003595 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Characterisation of Ribonuclease A Immobilized on Magnetic Cellulose - and Poly(2-Hydroxyethyl Methacrylate)-Based Supports
Original language description
A bioactive sorbent for degradation of high-molecular-weight RNA by RNase A was prepared by immobilization of RNase A on magnetic spherical carriers based on cellulose or poly(HEMA-co-EDMA). Immobilized RNase A was temperature-stable at 30-60 C. The influence of temperature on immobilized enzyme activity was also studied. A comparison of soluble and immobilized RNase A showed a pH shift in maximum of immobilized enzyme activity in dependence on the matrix type. Practical use of RNase A immobilized on magnetic bead cellulose MBC 1 for high-performance size-exclusion chromatography purification of pUC19 plasmid DNA is demonstrated.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EI - Biotechnology and bionics
OECD FORD branch
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Project
<a href="/en/project/GA203%2F98%2F1231" target="_blank" >GA203/98/1231: Oriented immobilization of enzymes and antibodies using biospecific interactions</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2000
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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