Characterisation of ribonuclease immobilised on cellulose and HEMA supports
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F99%3A00003568" target="_blank" >RIV/00216224:14310/99:00003568 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Characterisation of ribonuclease immobilised on cellulose and HEMA supports
Original language description
Chromatography is an integral part of large scale purification of plasmids. Samples usualy contains, except to cell components obtained after cell lysis, chromosomal DNA fragments, high molecular-weight RNA and plasmid variants. To achieve good chromatographic resolution it is necessary to degrade RNA by RNase. The aim of our work was to utilize RNase A immobilized on magnetic bead cellulose for degradation of high molecular-weight RNA before chromatographic purification of plasmid DNA. The enzyme was coupled to the particles via cyanuric chloride method. Temperature stability of soluble and immobilized RNase A was estimated after preincubation of the samples at different temperatures. Comparison of the pattern of soluble and immobilized RNase A showeda pH shift in activity. Practical use of magnetic particles in the purification of plasmid DNA is demonstrated.
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
EI - Biotechnology and bionics
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GA203%2F98%2F1231" target="_blank" >GA203/98/1231: Oriented immobilization of enzymes and antibodies using biospecific interactions</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
1999
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
13th International Symposium of Affinity Technology and Bio-Recognition
ISBN
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ISSN
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e-ISSN
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Number of pages
1
Pages from-to
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Publisher name
Université de Technologie de Compiegne
Place of publication
Compiegne
Event location
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Event date
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Type of event by nationality
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UT code for WoS article
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