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Electrochemical impedance spectroscopy of single-stranded and double-helical polynucleotides

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14330%2F00%3A00004092" target="_blank" >RIV/00216224:14330/00:00004092 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Electrochemical impedance spectroscopy of single-stranded and double-helical polynucleotides

  • Original language description

    The dependence of the impedance of the electrode double layer on time and frequency around the potentials of the tensammetric peaks and around the potential of maximum adsorption of single-stranded and double-helical polyadenylic acid (poly A) and DNA was studied. At room temperature the single stranded poly A (pH 8) yields a high tensammetric peak at about -1.4 V vs.SCE. The peak is higher and narrower at low ionic strengths. The height of the peak decreases with increasing frequency at low ionic strengths while it is almost independent on the frequency at higher ionic strengths. With double-helical (native ) DNA only one desorption maximum is observed on the C-E curves, single-stranded (denatured) DNA yields two maxima. It was shown by Miller and later by Palecek that the more positive desorption peak at -1.15 V yielded both by the native and denatured DNA corresponds to the desorption of the sugar - phospate backbone. The more negative peak at -1.4 V which yields only denatured DNA

  • Czech name

  • Czech description

Classification

  • Type

    D - Article in proceedings

  • CEP classification

    BO - Biophysics

  • OECD FORD branch

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2000

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Article name in the collection

    Book of Abstracts, J.Heyrovský Memorial Symposium, Praha

  • ISBN

  • ISSN

  • e-ISSN

  • Number of pages

    1

  • Pages from-to

    5

  • Publisher name

    Ústav fyzikální chemie J.Heyrovského, AV ČR

  • Place of publication

    Praha

  • Event location

    Praha

  • Event date

    Jan 1, 2000

  • Type of event by nationality

    WRD - Celosvětová akce

  • UT code for WoS article